Keratinolytic polypeptides and methods of use
AssigneeCornell University
InventorsXingen LEI, Madeleine KEEFE
Life of this patent
Estimating the term…
Abstract
Embodiments described herein relate to a recombinant nucleic acid construct comprising a nucleic acid molecule encoding a polypeptide having at least 90% sequence identity with the amino acid sequence, or fragment thereof, of SEQ ID NO:2; SEQ ID NO:4; SEQ ID NO; 6: SEQ ID NO:8; SEQ ID NO: 10; SEQ ID NO: 12; SEQ ID NO: 14; or SEQ ID NO: 16, as well as keratin-degrading compositions, methods of recombinantly producing a polypeptide, and methods of degrading keratin.
Claims24
A recombinant nucleic acid construct comprising:
- a nucleic acid molecule encoding a polypeptide having at least 90% sequence identity with the amino acid sequence, or fragment thereof, of SEQ ID NO:2;
- SEQ ID NO:4;
- SEQ ID NO:6;
- SEQ ID NO:8;
- SEQ ID NO:10;
- SEQ ID NO:12;
- SEQ ID NO:14;
- or SEQ ID NO:16.
17-19. (canceled)
A keratin-degrading composition comprising:
- a polypeptide having at least 90% sequence identity with the amino acid sequence, or fragment thereof, of SEQ ID NO:2, SEQ ID NO:4;
- SEQ ID NO:6;
- SEQ ID NO:8;
- SEQ ID NO:10;
- SEQ ID NO:12;
- SEQ ID NO:14;
- or SEQ ID NO:16, wherein the polypeptide is capable of degrading keratin or assisting in degrading keratin;
- and
- a carrier.
21-32. (canceled)
A method of recombinantly producing a polypeptide comprising:
- transforming a host cell with a heterologous nucleic acid molecule, wherein the nucleic acid molecule encodes a polypeptide having at least 90% sequence identity with the amino acid sequence, or fragment thereof, of SEQ ID NO:2;
- SEQ ID NO:4;
- SEQ ID NO:6;
- SEQ ID NO:8;
- SEQ ID NO:10;
- SEQ ID NO:12;
- SEQ ID NO:14;
- or SEQ ID NO:16.
34-44. (canceled)
47-50. (canceled)
Dependencies are inferred from the claim text.