Efficient biocompatible cryopreservation medium that eliminates the need for cell permeating cryoprotectants
AssigneesCryocrate LLC, The Curators of the University of Missouri
InventorsXu Han, Henry White, Peter Koulen
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Abstract
A cryopreservation medium including: a first cryoprotective particle or macromolecule: a second cryoprotective particle or macromolecule: and an aqueous liquid, wherein the first cryoprotective particle or macromolecule is hydrophilic and has a spherical shape when dissolved or suspended in the aqueous liquid, and wherein the second cryoprotective particle or macromolecule has an affinity for the first cryoprotective particle or macromolecule and an affinity for the plasma membrane of the cell.
Claims32
A cryopreservation medium comprising:
- a first cryoprotective particle or macromolecule;
- a second cryoprotective particle or macromolecule;
- and
- an aqueous liquid,
- wherein the first cryoprotective particle or macromolecule is hydrophilic and has a spherical shape when dissolved or suspended in the aqueous liquid, and
- wherein the second cryoprotective particle or macromolecule has an affinity for the first cryoprotective particle or macromolecule and an affinity for a plasma membrane of a cell or a lipid membrane of a lipid membrane bound biological structure.
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A method of protecting a lipid membrane of a lipid membrane bound biological structure, comprising:
- contacting the lipid membrane bound biological structure with a cryopreservation medium prior to cooling the lipid membrane bound biological structure to a temperature of about −70° C. to about −273° C.,
- wherein cubic ice is formed around the lipid membrane at the temperature of about −70° C. to about −273° C.
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Dependencies are inferred from the claim text.