Cluster segmentation and conditional base calling

A non-transitory computer readable storage medium comprising instructions that, when executed by at least one processor, cause a system to:

• segment a population of clusters into a plurality of subpopulations of clusters based on one or more prior bases called at one or more prior sequencing cycles of a sequencing run;
• and
• at a current sequencing cycle of the sequencing run:
• apply a mixture of four distributions to current sequenced data of each subpopulation of clusters in the plurality of subpopulations of clusters, wherein the four distributions correspond to four bases adenine (A), cytosine (C), guanine (G), and thymine (T), and wherein the current sequenced data is generated at the current sequencing cycle;
• and
• base call clusters in a particular subpopulation of clusters using a corresponding mixture of four distributions.

A computer-implemented method, including:

• segmenting a population of clusters into a plurality of subpopulations of clusters based on one or more prior bases called at one or more prior sequencing cycles of a sequencing run;
• and
• at a current sequencing cycle of the sequencing run:
• applying a mixture of four distributions to current sequenced data of each subpopulation of clusters in the plurality of subpopulations of clusters, wherein the four distributions correspond to four bases adenine (A), cytosine (C), guanine (G), and thymine (T), and wherein the current sequenced data is generated at the current sequencing cycle;
• and
• base calling clusters in a particular subpopulation of clusters using a corresponding mixture of four distributions.