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US 2024/0175876 A1Published application
Periplasmic ligand trapping system
AssigneeUniversity of Miami
InventorsDaniel Isom, Jacob Rowe
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Abstract
Disclosed herein are engineered yeast cells, systems, and methods for screening for a ligand that modulates a receptor function and for determining functional consequences of receptor-ligand binding.
Claims20
A yeast periplasmic ligand-trapping yeast display system comprising:
- a plurality of yeast cells, wherein each cell comprises:
- a recombinant expression construct encoding:
- a ligand;
- a cell-wall anchoring protein, wherein the ligand is fused to the cell-wall anchoring protein;
- and
- a signal sequence, wherein the signal sequence promotes secretion of the one or more ligands to the yeast cell periplasmic space;
- and
- an inducible reporter.
5 more elements
Dependencies are inferred from the claim text.