Nextgen chemometrics using proximity ligation assay (pla)

Abstract

The present invention relates to compositions and methods for material characterization using probe libraries. The methods include steps of unbiased sensing with probe libraries (e.g., oligonucleotide probes, or conjugated probes that comprise peptides conjugated to polynucleotide barcodes) that are followed with identifying informative patterns of the bounded probes when the probed libraries are applied to a material. In some examples, the probes used herein comprise binding moieties composed of random sequences (e.g., random peptide sequences or random polynucleotide sequences) that enable the unbiased sensing of the substrates in a sample for the determination of known and unknown materials. In some examples, the methods herein further comprise analyzing the sequences of the bounded oligonucleotide probes or the bounded conjugated probes and determining the patterns of the sequences that is indicative of the substrates bounded by the probes.

Dependencies are inferred from the claim text.