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US 2024/0175007 A1Published application
Compositions for and methods of improving directed evolution of biomolecules
OwnerErik Ali RODRIGUEZ, Sara MATTSON +3inventors
InventorsErik Ali RODRIGUEZ, Sara MATTSON, Felipe MONTECINOS-FRANJOLA, Nicholas CSIKAI, Leena ZITOUN
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Abstract
Disclosed herein are compositions for and methods of directing evolution of biomolecules. The disclosed methods simplify traditional error prone PCR of a single or multiple genes expressed on plasmid DNA for directed evolution and remove several previously required molecular biology steps, thereby affording facile purification of plasmid DNA expressing biomolecules directly from mammalian cells.
Claims23
A system for directed evolution of one or more biomolecules of interest, the system comprising:
- a DNA polymerase fused to a DNA binding protein, wherein the DNA polymerase lacks editing activity;
- and,
- a plasmid comprising one or more promoters for transcription and translation in a host cell, and one or more polynucleotide sequences encoding one or more biomolecules of interest to be evolved,
- wherein the system evolves the one or more biomolecules of interest by introducing one or more mutations into the one or more polynucleotide sequences encoding the one or more biomolecules of interest.
1 more element
Dependencies are inferred from the claim text.