Compositions comprising a variant polypeptide and uses thereof
AssigneeArbor Biotechnologies, Inc.
InventorsShaorong Chong, Brendan Jay Hilbert, Quinton Norman Wessells, Lauren E. Alfonse, Anthony James Garrity
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Abstract
The present invention relates to variant polypeptides, methods of preparing the variant polypeptides, processes for characterizing the variant polypeptides, compositions and cells comprising the variant polypeptides, and methods of using the variant polypeptides. The invention further relates to complexes comprising the variant polypeptides, methods of producing the complexes, processes for characterizing the complexes, cells comprising the complexes, and methods of using the complexes.
Claims87
A variant polypeptide comprising an alteration relative to a parent polypeptide of SEQ ID NO:
- 3, and wherein the alteration is a substitution of Table 2.
A variant polypeptide comprising an amino acid sequence having at least 95% identity to SEQ ID NO:
- 3 and comprising a substitution one or more of positions P14, E311, D32, 161, G223, N109, and D719 relative to SEQ ID NO:
- 3.
A variant polypeptide comprising an amino acid sequence having at least 95% identity to SEQ ID NO:
- 3 and comprising one or more of the following substitutions:
- P14R, E311R, D32R, 161R, G223R, N109R, and D719R.
A variant polypeptide comprising an amino acid sequence having at least 95% identity to SEQ ID NO:
- 3 and comprising a substitution one or more of positions K208, D302, D590, E154, D567, L38, D145, C13, T338, P14, D55, K221, K35, and E736 relative to SEQ ID NO:
- 3.
A variant polypeptide comprising an amino acid sequence having at least 95% identity to SEQ ID NO:
- 3 and comprising one or more of the following substitutions:
- K208G, D302G, D590G, E154G, D567G, L38G, D145G, C13G, T338G, P14G, D55G, K221G, K35G, and E736G.
The variant polypeptide or the composition of any previous claim, wherein the variant polypeptide further comprises a nuclear localization signal (NLS).
The variant polypeptide or the composition of any previous claim, wherein the variant polypeptide further comprises a peptide tag, a fluorescent protein, a base-editing domain, a DNA methylation domain, a histone residue modification domain, a localization factor, a transcription modification factor, a light-gated control factor, a chemically inducible factor, or a chromatin visualization factor.
A composition comprising a nucleic acid that encodes the variant polypeptide and/or the RNA guide of any previous claim.
The variant polypeptide or the composition of any previous claim, wherein the variant polypeptide is present in a delivery system comprising a nanoparticle (e.g., a lipid nanoparticle), a liposome, an exosome, a microvesicle, or a gene-gun.
A cell comprising the variant polypeptide or the composition of any previous claim.
A composition comprising a variant polypeptide or a complex comprising the variant polypeptide, wherein the variant polypeptide comprises an alteration relative to a parent polypeptide of SEQ ID NO:
- 3, and wherein the variant polypeptide or the complex exhibits enhanced enzymatic activity, enhanced binding activity, enhanced binding specificity, and/or enhanced stability, relative to a parent polypeptide or a complex comprising the parent polypeptide.
A method for editing a gene in a cell, the method comprising contacting the cell with the variant polypeptide or composition of any one of the previous claims.
Dependencies are inferred from the claim text.