Method for producing ovarian somatic cell-like cells, and method for inducing differentiation of primate pluripotent stem cells into ovarian somatic cell-like cells

Abstract

A method for producing ovarian somatic cell-like cells includes (1) culturing primate pluripotent stem cells in a medium including a GSK3 inhibitor and a ROCK inhibitor; (2) culturing the cells from the (1) culturing in a medium including BMP4, retinoic acid, and an MEK inhibitor; and (3) culturing the cells from the (2) culturing in a base medium to obtain ovarian somatic cell-like cells. A method for inducing the differentiation of primate pluripotent stem cells into ovarian somatic cell-like cells includes inducing the differentiation of primate pluripotent stem cells into ovarian somatic cell-like cells using the method for producing ovarian somatic cell-like cells.

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