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US 2024/0174978 A1Published application
Modalities for the treatment of degenerative diseases of the retina
AssigneeAstellas Institute for Regenerative Medicine
InventorIrina Klimanskaya
Life of this patent
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Abstract
This invention relates to methods for improved cell-based therapies for retinal degeneration and for differentiating human embryonic stem cells and human embryo-derived into retinal pigment epithelium (RPE) cells and other retinal progenitor cells.
Claims18
1-18. (canceled)
A method for the spontaneous differentiation of human pluripotent stem cells into RPE cells, said method comprising:
- (a) providing a multilayer of human pluripotent stem cells;
- (b) culturing the multilayer of cells in media lacking exogenously added basic FGF, LIF and Plasmanate for a sufficient time for the appearance of pigmented cells;
- and
- (c) isolating and culturing the pigmented cells from the culture of (b), thereby obtaining RPE cells having a cobblestone, polygonal, epithelial-like appearance and that are bestrophin+.
1 more element
A method for the spontaneous differentiation of human pluripotent stem cells into RPE cells, said method comprising:
- (a) providing embryoid bodies formed from human pluripotent stem cells;
- (b) culturing said embryoid bodies in media lacking exogenously added basic FGF, LIF and Plasmanate for a sufficient time for the appearance of pigmented cells;
- and
- (c) culturing the pigmented cells from the cell culture of (b), thereby obtaining RPE cells having a cobblestone, polygonal, epithelial-like appearance and that are bestrophin+.
1 more element
Dependencies are inferred from the claim text.