Method for operating a bioprocess installation

A bioprocess installation with an electronic process control and at least one bioprocess unit, wherein the bioprocess unit comprises a cell broth source with a first receptacle for cell broth including cultivation media and cells, establishing a culture environment for cell cultivation and/or bioproduction, wherein the bioprocess unit comprises a clarification setup with a centrifuge for the clarification of the cell broth by centrifugation, with a liquid pumping arrangement assigned to the centrifuge and with a liquid network with a number of liquid lines communicating with the liquid pumping arrangement, wherein out of a first culture environment established by the first receptacle, the cell broth is transferred to the centrifuge via the liquid network, which centrifuge is operated in a forward operation for cell separation and/or cell washing and a backward operation for cell discharging,

• wherein the bioprocess installation comprises a valve arrangement, which allows to deactivate and activate at least one of the liquid lines,
• wherein the liquid network comprises a recycling line and that in the backward operation, at least part of the discharged cells may be transferred into a second culture environment different from the first culture environment via the recycling line for subsequent cell cultivation and/or bioproduction.