Trypsin-like serine proteases and uses thereof
AssigneeDanisco US Inc.
InventorsLilia M Babe, Xiaogang Gu, Thijs Kaper
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Abstract
Described herein is at least one novel trypsin-like serine protease polypeptide and uses thereof. Further described herein are cleaning compositions containing at least one polypeptide described herein, wherein said composition can be used to clean fabrics and hard surfaces. Even further described herein is at least one cleaning composition selected from a laundry detergent, a dishwashing detergent (e.g., automatic and hand dish), and a personal care composition. Even still further, at least one polypeptide having improved soil removal and/or stability compared to at least one reference polypeptide is described herein.
Claims23
An isolated polypeptide or active fragment thereof having serine protease activity, wherein the polypeptide comprises an amino acid sequence having at least 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity with an amino acid sequence selected from the group consisting of SEQ ID NO:
- 7, 8, and 13.
An isolated polypeptide having serine protease activity, wherein the polypeptide comprises an amino acid sequence having at least 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity with an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 7, 8, and 13. 3 A recombinant construct comprising a regulatory sequence functional in a production host operably linked to a nucleotide sequence encoding at least one polypeptide having an amino acid sequence having at least 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity with an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 7, 8, and 13.
The recombinant construct of claim 3, wherein said host is selected from the group consisting of fungi, bacteria, and algae.
A host cell comprising a recombinant nucleic acid construct comprising an isolated polynucleotide comprising a nucleotide sequence that encodes a polypeptide having serine protease activity, wherein the polypeptide has at least 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity with an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 7, 8, and 13operably linked to a promoter sequence capable of controlling expression of the polynucleotide sequence. 6 The host cell of claim 5, wherein said host is selected from the group consisting of fungi, bacteria, and algae.
A method for producing at least one polypeptide comprising:
- (a) transforming a production host with the recombinant construct comprising a regulatory sequence functional in a production host operably linked to a nucleotide sequence encoding at least one polypeptide having an amino acid sequence having at least 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity with an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 7, 8, and 13;
- and
- (b) culturing the production host of step (a) under conditions whereby at least one polypeptideis produced.
A cleaning or detergent composition comprising at least one polypeptide having an amino acid sequence having at least 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity with an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 7, 8, and 13and at least one surfactant.
A cleaning or detergent composition comprising at least one polypeptide having an amino acid sequence having at least 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity with an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 7, 8, and 13and at least one dispersant or dispersing polymer.
The cleaning or detergent composition of claims 16-18, wherein the surfactant is a nonionic alcohol ethoxylate. 20 The cleaning or detergent composition of claims 16-19, further comprising a second polypeptide having protease activity;
- and, optionally, at least one additional polypeptide, wherein the at least one additional polypeptide is an enzyme selected from the group consisting of:
- acyl transferases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinosidases, aryl esterases, beta-galactosidases, carrageenases, catalases, cellobiohydrolases, cellulases, chondroitinases, cutinases, endo-beta-1,4-glucanases, endo-beta-mannanases, esterases, exo-mannanases, feruloyl esterase, galactanases, glucoamylases, hemicellulases, hexosaminidases, hyaluronidases, keratinases, laccases, lactases, ligninases, lipases, lipoxygenases, mannanases, metalloproteases, nucleases (e.g. deoxyribonucleases and ribonucleases), oxidases, oxidoreductases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, perhydrolases, peroxidases, phenoloxidases, phosphatases, phospholipases, phytases, polygalacturonases, polyesterases, proteases, pullulanases, reductases, rhamnogalacturonases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, xyloglucanases, xylosidases, and any combination or mixture thereof.
A method of cleaning, comprising contacting a surface or an item in need of cleaning with an effective amount of at least one polypeptide having serine protease activity, wherein the polypeptide comprises an amino acid sequence having at least 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity with an amino acid sequence selected from the group consisting of SEQ ID NO:
- 7, 8, and 13;
- and optionally further comprising the step of rinsing said surface or item after contacting said surface or item with said polypeptide.
A method of cleaning, comprising contacting a surface or an item in need of cleaning with a cleaning or detergent composition comprising at least one polypeptide having an amino acid sequence having at least 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity with an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 7 and 87, 8, and 13 and at least one surfactant or dispersant polymer;
- and optionally further comprising the step of rinsing said surface or item after contacting said surface or item with said composition.
Dependencies are inferred from the claim text.