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US 2024/0174720 A1Published application
Alkali-Tolerant Affinity Chromatography Ligands
AssigneeGuangzhou Binding Technologies Co., Limited
InventorYinong Zong
Life of this patent
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Abstract
This disclosure relates to chromatography ligands comprising one or more domains of Staphylococcal Protein A (SPA), or any functional variants thereof, as well as methods of making and using the same.
Claims21
-27. (canceled)
A polypeptide comprising a first immunoglobulin (Ig)-binding domain, wherein the first Ig-binding domain comprises:
- (1) the amino acid sequence that is at least 90% identical to SEQ ID NO:
- 1, wherein the amino acid that corresponds to N55 of SEQ ID NO:
- 1 is hydrophobic;
- (2) the amino acid sequence that is at least 90% identical to SEQ ID NO:
- 2, wherein the amino acid that corresponds to N54 of SEQ ID NO:
- 2 is hydrophobic;
- (3) the amino acid sequence that is at least 90% identical to SEQ ID NO:
- 3, wherein the amino acid that corresponds to N52 of SEQ ID NO:
- 3 is hydrophobic;
- (4) the amino acid sequence that is at least 90% identical to SEQ ID NO:
- 4, wherein the amino acid that corresponds to N52 of SEQ ID NO:
- 4 is hydrophobic;
- (5) the amino acid sequence that is at least 90% identical to SEQ ID NO:
- 5, wherein the amino acid that corresponds to N52 of SEQ ID NO:
- 5 is hydrophobic;
- or
- (6) the amino acid sequence that is at least 90% identical to SEQ ID NO:
- 6, wherein the amino acid that corresponds to N52 of SEQ ID NO:
- 6 is hydrophobic.
16 more elements
A polypeptide comprising an immunoglobulin (Ig)-binding domain, wherein the Ig-binding domain comprises SEQ ID NO:
- 6 with a mutation at N52, wherein N52 is substituted with a hydrophobic amino acid residue.
Dependencies are inferred from the claim text.