Back to results
US 2024/0174709 A1Published application
Nucleotide analogues
AssigneeSingular Genomics Systems, Inc.
InventorsRonald Graham, Olga Adelfinskaya, Megha Cila, Rodrigo Rodriguez, Abrehet Abdu, Eli N. Glezer
Life of this patent
Estimating the term…
Abstract
Disclosed herein, inter alia, are compounds, compositions, and methods of using the same for the sequencing of a nucleic acid.
Claims21
-40. (canceled)
A method of sequencing a nucleic acid molecule comprising:
- (a) incorporating in series a plurality of nucleotides into a primer hybridized to the nucleic acid molecule to create an extension strand, wherein each nucleotide comprises:
- (i) a cleavable moiety covalently bound to the 3′ oxygen of the nucleotide, said cleavable moiety having the formula -L1P-S—S—R6P (I);
- wherein, L1P is a substituted methylene, wherein L1P is substituted with a substituted or unsubstituted alkyl, or a substituted or unsubstituted heteroalkyl;
- R1P is unsubstituted C1-C4 alkyl;
- and
- (ii) a detectable moiety attached to said nucleotide via a covalent linker, wherein said covalent linker comprises
- wherein, R102 is unsubstituted C1-C4 alkyl;
- and
- (b) detecting the detectable label of the incorporated nucleotide to identify the incorporated nucleotide in the extension strand, thereby sequencing the nucleic acid molecule.
6 more elements
Dependencies are inferred from the claim text.