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US 2024/0173719 A1Published application
Devices and methods for rapid sample processing and analysis
AssigneeNorthwestern University
InventorsSally Maureen MCFALL, David M. Kelso, Jennifer Lynn Reed, Tom Westberg
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Abstract
Provided herein are devices and methods for the rapid processing and analysis of samples. In particular, a small-volume sample (e.g., nucleic acid sample) is exposed to (e.g., contacted with) different temperature zones within a device to process (e.g., amplify) and/or analyze (e.g., quantitate) the sample in an assay.
Claims9
A method of thermocycling a sample comprising:
- (a) placing the sample in the sample container in a cassette, wherein the cassette comprises a sample container of 1 mm or less thickness sandwiched between front and rear surfaces of 1 mm or less thickness each;
- (b) holding the sample container in a stationary position while cycling the sample container through first and second temperature zones multiple times;
- wherein the first temperature zone is achieved by placing a first pair of heaters in opposing orientations on either side of the sample container adjacent to the front and rear surfaces, respectively;
- wherein the second temperature zone is achieved by placing a second pair of heaters in opposing orientations on either side of the sample container adjacent to the front and rear surfaces, respectively.
1 more element
Dependencies are inferred from the claim text.