Limiting viral infection and replication in stem cells
AssigneeGeorge Mason University
InventorsYuntao Wu, Sijia He, Deemah Dabbagh
Life of this patent
Estimating the term…
Abstract
An Embodiment relates to a method comprising: co-expressing a viral vector of a virus of interest and a CD34 expression vector into a human cell line to produce virus particles such that CD34 incorporates into the virus particles; isolating the virus particles from the human cell line; blocking a viral infection; and wherein the method is configured for inhibiting the viral infection of the virus of interest.
Claims26
An in vitro method comprising:
- co-expressing a viral vector of a virus of interest and a CD34 expression vector into a human cell line to produce virus particles such that CD34 incorporates into the virus particles;
- isolating the virus particles from the human cell line;
- blocking a viral infection;
- and wherein the method is configured for inhibiting the viral infection of the virus of interest.
(canceled)
A composition comprising a recombinant virus particle comprising an overexpressed CD34 protein;
- wherein the recombinant virus particle comprises a non-infectious virus particle, an attenuated virus particle or an inactivated virus particle or a combination thereof.
(canceled)
(canceled)
17-18. (canceled)
An ex vivo method comprising:
- taking CD34+ cells;
- transfecting a viral vector expressing a virus of interest into the CD34+ cells;
- producing virus particles in the CD34+ cells and isolating the virus particles from the CD34+ cells, and wherein the virus particles are non-infectious virus particles, attenuated virus particles or inactivated virus particles or a combination thereof of the virus of interest.
24-25. (canceled)
28-30. (canceled)
Dependencies are inferred from the claim text.