Biological sample preparation and analysis
AssigneeDH Technologies Development Pte. Ltd.
InventorsAaron Stella, Chang Liu, Yuguan Liu, Subhasish Purkayastha
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Abstract
Methods and kits for preparing liquid samples are presently claimed and described. The method may include treating liquid sample with a hydrolysis enzyme, hydrolyzing the liquid sample to prepare a hydrolysate, and purifying the hydrolysate with magnetic based purification. In certain aspects, the hydrolysis enzyme is bound to a magnetic bead or a magnetic particle. Kits for preparing a liquid sample can include a hydrolysis enzyme, magnetic beads or magnetic particles, one or more internal standards, a liquid chromatography column and one or more solvents to be used as mobile phases, one or more calibrant solutions and instructions for use.
Claims34
A method of preparing a liquid sample, comprising:
- treating the liquid sample with a hydrolysis enzyme,
- hydrolyzing the liquid sample to prepare a hydrolysate, and
- purifying the hydrolysate with magnetic based purification.
A kit for preparing a liquid sample, wherein the kit comprises:
- a hydrolysis enzyme selected from the group consisting of β-glucuronidase, trypsin, chymotrypsin, proteases, LysC, LysN, AspN, GluC, ArgC, pronase, pepsin, prolidase, and a biotinylated enzyme, and
- magnetic beads or magnetic particles, wherein the magnetic beads or magnetic particles comprise a monoclonal antibody, a polyclonal antibody, streptavidin, or have a surface coating selected from the group consisting of dextran, sodium carboxylate, phosphate, diphosphate, polyacrylic acid, oleic acid, silica, 2-hydroxypropyl trimethylammonium chloride, polyethylenimine, diethylaminoethyl cellulose, poly(4-vinylpyridine), and sodium sulfonate,
- one or more internal standards, a liquid chromatography column, and one or more solvents to be used as mobile phases, one or more calibrant solutions, and instructions for use.
Dependencies are inferred from the claim text.