Method for rna manufacturing
AssigneeeTheRNA immunotherapies NV
InventorsSenne DILLEN, Lore DE BRUYNE, Michiel HOLTOF
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Abstract
The present invention relates to the field of nucleic acid production, in particular in vitro RNA transcription. More specifically, the present invention relates to a method wherein physicochemical properties of the reaction mixture are monitored in order to timely stop the reaction and/or to add fresh reagents to allow the reaction to continue under optimal conditions. In particular, conductivity, but also pH and/or optical density is determined at at least two different time points, or continuously during the in vitro transcription reaction.
Claims10
A method for manufacturing RNA using an in vitro transcription reaction comprising determining the conductivity during said transcription reaction at at least two different time points, wherein upon detecting a decrease in conductivity between said at least two time points, the reaction is stopped or supplemented with fresh reagents.
Use of conductivity measurements during an in vitro transcription reaction in the manufacturing of RNA.
Dependencies are inferred from the claim text.