Method for improving efficiency of genetic transformation and gene editing in plants
AssigneeSuzhou Qi Biodesign Biotechnology Company Limited
InventorsCaixia Gao, Lu Chen
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Abstract
The invention belongs to the field of genetic engineering in plants. In particular, the invention relates to a method for improving efficiency of genetic transformation and gene editing in plants. More particularly, the invention relates to a method for improving regeneration efficiency of genetic transformation and/or gene editing efficiency in plants by expression of gene NiR which promotes nitrogen metabolism in plants.
Claims16
A method of improving regeneration efficiency of a plant cell, comprising:
- (a) overexpressing NiR in the plant cell, such as introducing an expression construct containing a coding nucleic acid sequence of NiR into the plant cell;
- (b) regenerating into an intact plant from the plant cell.
A method of transforming at least one exogenous nucleic acid sequence of interest into a plant, comprising:
- (a) overexpressing NiR in a cell of the plant, such as introducing an expression construct containing a coding nucleic acid sequence of NiR into the cell of the plant;
- (b) introducing at least one expression construct containing at least one exogenous nucleic acid sequence of interest into the plant cell;
- and
- (c) regenerating into an intact plant from the plant cell.
A method of gene editing in a plant, comprising:
- (a) overexpressing NiR in a cell of the plant, such as introducing an expression construct containing a coding nucleic acid sequence of NiR into the cell of the plant;
- (b) introducing at least one expression construct containing at least one exogenous nucleic acid sequence of interest into the plant cell, wherein the at least one exogenous nucleic acid sequence of interest encodes a component of the gene editing system;
- (c) regenerating into an intact plant from the plant cell.
Dependencies are inferred from the claim text.