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US 2024/0158756 A1Published application
Feeder-based and feeder-free stem cell culture systems for stratified epithelial stem cells, and uses related thereto
AssigneeUniversity of Houston System
InventorsFrank McKEON, Wa XIAN, Matthew P. VINCENT, Jingzhong XIE, Wei RAO
Life of this patent
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Abstract
The present invention relates to a culture media system that is useful for the isolation and epigenetically stable propagation of normal stem cells in culture, wherein the stem cells are derived from stratified epithelial tissues and cancer stem cells from epithelial cancers. In certain embodiments, the culture system is a feeder-free system.
Claims7
A defined culture medium for isolating and stably maintaining the epigenetics of stratified epithelial stem cells through a plurality of passaging in culture, the medium comprising:
- a basal medium;
- and each of a ROCK (Rho Kinase) inhibitor, a mitogenic growth factor, insulin or IGF, a TrkA Inhibitor, and an Oct4-activating agent, which culture media includes at least one of a VEGF inhibitor, a tyrosine kinase inhibitor and/or FGF10 or an FGF10 agonist, and which culture media optionally further includes a TGFβ signaling pathway inhibitor, and/or a Bone Morphogenetic Protein (BMP) antagonist, wherein the culture media supports the epigenetically stable growth and proliferation of stem cells of stratified epithelial tissue origin in culture.
Dependencies are inferred from the claim text.