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US 2024/0158751 A1Published application
Methods for Storing Hematopoietic Stem Cells
AssigneeHemanext Inc.
InventorSamuel O. SOWEMIMO-COKER
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Abstract
The present disclosure relates to devices and methods for the improved storage and expansion of stem cells.
Claims25
A method for preparing stem cells for transplantation into a patient in need thereof comprising:
- collecting a blood product containing stem cells into an oxygen absorbing environment comprising an hypoxic collection container comprising an O2 barrier characterized by an oxygen (O2) permeability of less than 0.5 cc of oxygen per square meter per day and an oxygen sorbent;
- mixing the blood product until the initial partial pressure of oxygen (pO2) of the blood product is reduced by between 80 and 95% separating leukocytes and stem cells from said blood product comprising applying said blood product to a filter wherein the stem cells and leukocytes are retained on said filter to prepare a leukocyte and stem cells depleted blood product;
- eluting said stem cells from said filter with an isotonic media;
- and
- transferring said stem cells into a hypoxic storage container comprising an O2 barrier characterized by an oxygen (O2) permeability of less than 0.5 cc of oxygen per square meter per day and storing said cells to prepare hypoxic stored stem cells.
2 more elements
A method for preparing stem cells for transfusion comprising:
- collecting a blood product comprising stem cells;
- separating leukocytes and stem cells from said blood product;
- transferring said stem cells into a hypoxic storage container comprising an O2 barrier characterized by an oxygen (O2) permeability of less than 0.5 cc of oxygen per square meter per day, and
- storing said stem cells in said hypoxic storage container at a partial pressure of oxygen of less than 3500 Pa for a period of time at a temperature of less than 37° C. to form a hypoxic storage bag comprising frozen hypoxic stored stem cells.
1 more element
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A method of preparing stem cells for transplantation comprising exposing an hypoxic storage bag comprising frozen hypoxic stored stem cells to at least 25° C. to form thawed hypoxic stored stem cells;
- centrifuging said thawed hypoxic stored stem cells and removing the supernatant;
- and
- resuspending said hypoxic stored stem cells in a solution comprising 3% dextran 40, wherein the hypoxic storage bag comprises a hypoxic environment of less than 3500 Pa.
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Dependencies are inferred from the claim text.