Biosynthesis of mogrosides
AssigneeGinkgo Gioworks, Inc.
InventorsDiveena Becker, Josef Bober, Justin Michael Gardin, Matthew McMahon
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Abstract
Described in this application are proteins and host cells involved in methods of producing mogrol precursors, mogrol, and/or mogrosides.
Claims46
A host cell that comprises a heterologous polynucleotide encoding a cytochrome b5 (CB5), wherein the host cell is capable of producing more mogrol than a control host cell that 5 does not comprise the heterologous polynucleotide, and wherein the CB5 comprises:
- a) the amino acid sequence
- (SEQ ID NO:
- 47)
- YTGLSP;
- b) the amino acid sequence
- (SEQ ID NO:
- 48)
- KPLLMAIKGQIYDVS;
- c) the amino acid sequence
- (SEQ ID NO:
- 49)
- LQDWEYKFM;
- and/or
- d) the amino acid sequence
- (SEQ ID NO:
- 53)
- X1X2X3EX4GX5X6X7X8X9X10D,
- wherein:
- (i) X1 is the amino acid K or E;
- (ii) X2 is the amino acid P or H;
- (iii) X3 is the amino acid A or S;
- (iv) X4 is the amino acid D or N;
- (v) X5 is the amino acid P or H;
- (vi) X6 is the amino acid S or R;
- (vii) X7 is the amino acid E or N;
- (viii) X8 is the amino acid S or F;
- (ix) X9 is the amino acid Q or E;
- and/or
- (x) X10 is the amino acid A or I.
A host cell that comprises a heterologous polynucleotide encoding a cytochrome b5 (CB5), wherein the CB5 comprises a sequence that is at least 90% identical to any one of SEQ ID NOs:
- 1-10 and 318 and wherein the host cell is capable of producing mogrol.
A host cell that comprises a heterologous polynucleotide encoding a cytochrome b5 (CB5), wherein the CB5 comprises a sequence that is at least 90% identical to any one of SEQ ID NOs:
- 1-4 and 318 and wherein the host cell is capable of producing more mogrol than a control host cell that does not comprise the heterologous polynucleotide.
A host cell that comprises a heterologous polynucleotide encoding a cytochrome b5 (CB5), wherein the heterologous polynucleotide comprises a sequence that is at least 90% identical to any one of SEQ ID NOs:
- 11-24, 316-317, and 330-331, and wherein the host cell is capable of producing mogrol.
A host cell that comprises a heterologous polynucleotide encoding a cytochrome b5 (CB5), wherein the CB5 comprises:
- a) the amino acid sequence
- (SEQ ID NO:
- 54)
- ILRVSFRKYRKAIEQ;
- b) the amino acid sequence
- (SEQ ID NO:
- 55)
- RAFRPSIRFKKSHSTVPT;
- c) the amino acid sequence
- (SEQ ID NO:
- 56)
- KNTLYVGG;
- and/or
- d) the amino acid sequence
- (SEQ ID NO:
- 57)
- DQATQKHRSFGFVTFLEKED
- and wherein the host cell is capable of producing more mogrol than a control host cell that does not comprise the heterologous polynucleotide.
A non-naturally occurring polynucleotide comprising a sequence that is at least 90% identical to any one of SEQ ID NOs:
- 11-14, 22-24, 316-317, and 330-331.
Dependencies are inferred from the claim text.