Fungal protease mixtures and uses thereof
AssigneeBio-Cat, Inc.
InventorsSean Michael GARVEY, Kelly Marie TINKER, Justin Lamont GUICE, Robert Daniel LITTLE, Christopher Edward SCHULER
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Abstract
Fungal protease compositions, and more particularly, proteolytic enzyme mixtures comprising a plurality of Aspergillus proteases are provided. The disclosure also relates to protein hydrolysates, food and beverage products and dietary supplements produced using these proteolytic enzyme mixtures, and methods of making and using the same.
Claims21
A proteolytic enzyme mixture comprising a plurality of fungal proteases from members of the genus Aspergillus, wherein the plurality of fungal proteases comprises:
- (a) a 42 kDa protease with exo- and endo-protease activity obtained from A. oryzae, (b) a 34 kDA protease with peptidase activity obtained from A. melleus, and (c) a 35 kDa fungal neutral protease obtained from A. oryzae, and the proteolytic enzyme mixture has exoprotease, endoprotease and peptidase activity.
A proteolytic enzyme mixture comprising a plurality of fungal proteases from the genus Aspergillus,
- wherein the mixture comprises at least two of SEQ ID NOs:
- 1, 2, and 3;
- or
- wherein the mixture includes at least one variant of SEQ ID NOs:
- 1, 2, or 3, comprising:
- a) an enzyme which shares at least 90, 91, 92, 93, 94, 95, 96, 97, 98 or 99% full-length sequence identity with any one of SEQ ID NOs:
- 1-3;
- b) an enzyme which shares at least 90, 91, 92, 93, 94, 95, 96, 97, 98 or 99% full-length sequence identity with the region spanning position 78-404 of SEQ ID NO:
- 1;
- c) an enzyme which shares at least 90, 91, 92, 93, 94, 95, 96, 97, 98 or 99% full-length sequence identity with the region spanning position 246-634 of SEQ ID NO:
- 3;
- or
- d) any combination of a), b) and/or c);
- wherein the variant retains the enzymatic activity of any one of SEQ ID NOs:
- 1-3.
(canceled)
Dependencies are inferred from the claim text.