Glycosylated Opioids
AssigneeRiver Stone Biotech Aps
InventorsJens Houghton-Larsen, Rubini Kannangara, Esben Halkjaer Hansen, Evan Chaberski, Laura Tatjer-Recordá
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Abstract
The present invention relate to a method for producing an oripavine glycoside and/or nororipavine glycoside comprising providing (i) a oripavine acceptor and/or nororipavine acceptor, (ii) a glycosyl donor, and (iii) a glycosyl transferase (UGT), and contacting the oripavine acceptor and/or nororipavine acceptor, the glycosyl donor, and the UGT at conditions allowing the UGT to transfer a glycosyl moiety from the glycosyl donor to the oripavine acceptor and/or nororipavine acceptor and thereby produce the oripavine glycoside and/or nororipavine glycoside.
Claims103
A method for producing an oripavine glycoside and/or nororipavine glycoside comprising providing (i) a oripavine acceptor and/or nororipavine acceptor, (ii) a glycosyl donor, and (iii) a glycosyl transferase (UGT), and contacting the oripavine acceptor and/or nororipavine acceptor, the glycosyl donor, and the UGT at conditions allowing the UGT to transfer a glycosyl moiety from the glycosyl donor to the oripavine acceptor and/or nororipavine acceptor and thereby produce the oripavine glycoside and/or nororipavine glycoside.
A method for producing an oripavine aglycone and/or nororipavine aglycone comprising providing (i) a oripavine glycoside and/or nororipavine glycoside and (ii) a glycosidase and contacting the oripavine glycoside and/or nororipavine glycoside and (ii) with the glycosidase at conditions allowing the glycosidase to catalyze separation of a glycosyl moiety from the oripavine glycoside and/or nororipavine glycoside and thereby produce the oripavine aglycone and/or nororipavine aglycone.
A glycoside comprising an oripavine aglycone and/or nororipavine aglycone and a glycosyl group.
A microbial host cell genetically modified to produce an oripavine glycoside and/or nororipavine glycoside in the presence of a glycosyl donor, wherein the host cell expresses one or more heterologous genes encoding one or more UGT's, which in the presence of a glycosyl donor and a oripavine acceptor and/or nororipavine acceptor, transfers a glycosyl moiety from the glycosyl donor to the oripavine acceptor and/or nororipavine acceptor and thereby produce the oripavine glycoside and/or nororipavine glycoside.
Dependencies are inferred from the claim text.