Tobacco plants exhibiting altered photosynthesis and methods of making and using
AssigneeAltria Client Services LLC
InventorsYanxin SHEN, Dongmei XU
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Abstract
This disclosure provides tobacco plants that exhibit altered photosynthesis as well as methods of making and using such plants.
Claims21
1-21. (canceled)
A method of making a Nicotiana tabacum plant, comprising:
- selecting a Nicotiana tabacum plant comprising a non-natural mutation in an endogenous nucleic acid sequence encoding a polypeptide having at least 95% sequence identity to a sequence selected from the group consisting of SEQ ID NOs:
- 22, 24, and 26;
- crossing or selfing said selected Nicotiana tabacum plant to produce at least one progeny seed;
- and
- obtaining at least one progeny Nicotiana tabacum plant grown from said at least one progeny seed, wherein said at least one progeny Nicotiana tabacum plant comprises said non-natural mutation;
- and
- wherein said at least one progeny Nicotiana tabacum plant comprising said non-natural mutation has reduced expression or activity of a polypeptide having at least 95% sequence identity to a sequence selected from the group consisting of SEQ ID NOs:
- 22, 24, and 26 compared to a control tobacco plant lacking said non-natural mutation.
A Nicotiana tabacum plant, or part thereof, comprising an altered photosynthesis phenotype and reduced expression or activity of a polypeptide having at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs:
- 22, 24, and 26;
- wherein said altered photosynthesis phenotype is selected from the group consisting of a reduced concentration of thylakoid membranes in the photosystems, a reduced amount of total chlorophyll, and an increased ratio of chlorophyll a to chlorophyll b;
- wherein said reduced or increased amounts or ratio are in comparison to a Nicotiana tabacum plant having wild-type activity of said polypeptide.
A Nicotiana tabacum plant, or part thereof, comprising a non-natural mutation in an endogenous nucleic acid, the wild type endogenous nucleic acid encoding a polypeptide sequence having at least 95% identity to a sequence selected from the group consisting of SEQ ID NOs:
- 22, 24, and 26;
- wherein said Nicotiana tabacum plant comprising said non-natural mutation has reduced expression or activity of said polypeptide sequence.
Dependencies are inferred from the claim text.