Novel agrobacterium with no overgrowth in plant transformation
OwnerShujun Chang, Sunilkumar Ganesan +1inventors
InventorsShujun Chang, Sunilkumar Ganesan, Fred Moshiri
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Abstract
The instant disclosure provides a genetically modified bacterial cell, i.e., modified Agrobacterium strain, which expresses a levansucrase and has reduced expression of endogenous CysK. As a result, the growth of the modified bacterial cell on medium or tissue culture with sucrose and deficient in cysteine is inhibited. The two-action growth control of the modified Agrobacterium strain can overcome the unmet issue of Agrobacterium overgrowth during plant transformation, and therefore increase the efficiency of plant transformation.
Claims39
A modified bacterial cell comprising a heterologous polynucleotide comprising a nucleic acid sequence encoding a levansucrase enzyme, wherein an endogenous CysK gene has reduced expression when compared to a proper control bacterium.
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A polynucleotide comprising from the 5′ to 3′:
- (a) a 5′ fragment of a CysK coding sequence;
- (b) a levansucrase coding sequence;
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- (c) a 3′ fragment of the CysK coding sequence.
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Dependencies are inferred from the claim text.