Production of fatty acid derivatives
AssigneeGenomatica, Inc.
InventorsDerek L. GREENFIELD, Andreas W. Schirmer, Elizabeth J. Clarke, Eli S. Groban, Bernardo M. Da Costa, Zhihao Hu, Kevin Holden, Noah Helman
Life of this patent
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Abstract
The disclosure relates to recombinant host cells including strain modifications effective to improve titer, yield and/or productivity of fatty acid derivatives. The disclosure further relates to cell cultures including the recombinant host cells for the fermentative production of fatty acid derivatives and compositions thereof.
Claims21
-51. (canceled)
A recombinant bacterial host cell expressing one or more polypeptides having one or more of 3-hydroxy-acyl-[acp] dehydratase activity, β-ketoacyl-ACP synthase I activity, β-ketoacyl-ACP synthase II activity, β-ketoacyl-ACP synthase III activity, enoyl-ACP reductase activity, [acp] S-malonyltransferase activity, 3-oxoacyl-{β-ketoacyl}-ACP reductase activity, or trans-2, cis-3-decenoyl-ACP isomerase activity;
- wherein the recombinant bacterial host cell produces a fatty acid derivative composition at a higher titer, yield, or productivity than a corresponding wild type host cell when cultured in a medium containing a carbon source.
A recombinant host cell comprising a genetically engineered polynucleotide sequence comprising a transposon, wherein insertion of said transposon into ayijP gene affects a second gene flanking said yjP gene, wherein said second gene codes for a polynucleotide that is up- or down regulated, and wherein said up- or down regulated polynucleotide codes for a polypeptide that affects production of a fatty acid derivative composition when said host cell is cultured in a medium containing a carbon source under conditions effective to express said polypeptide.
Dependencies are inferred from the claim text.