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US 2024/0141403 A1Published application
Methods for Producing Recombinant Proteins
AssigneeThe Regents of the University of California
InventorsAlanna Schepartz Shrader, Sebasthian Santiago González
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Abstract
Recombinant proteins comprising a non-canonical amino acid with high yield and high fidelity are made by expressing the protein in an engineered Vibrio natriegens strain containing an orthogonal translation system comprising an orthogonal aminoacyl tRNA synthetase that charges the non-canonical amino acid onto the orthogonal cognate tRNA.
Claims20
A method of producing a recombinant protein comprising a non-canonical amino acid (ncAA) with high yield and high fidelity, the method comprising:
- expressing the protein in an engineered Vibrio natriegens strain comprising an orthogonal translation system (OTS) comprising an orthogonal aminoacyl tRNA synthetase (aaRS) that charges the non-canonical amino acid onto a cognate, orthogonal tRNA.
Dependencies are inferred from the claim text.