Systems and methods for protein expression

A composition comprising a vector system comprising one or more vectors, the one or more vectors, comprising:

• a) a first polynucleotide encoding an amino acid sequence from Table 8 or Table 9;
• and
• b) a second polynucleotide encoding a picornavirus leader (L) protein with an amino acid sequence selected from the group consisting of SEQ ID NOS:
• 1-6, and 24, or an amino acid sequence with at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity thereto;
• wherein the first polynucleotide encoding the amino acid sequence from Table 8 or Table 9, and the second polynucleotide encoding the L protein are operatively linked to one or more promoters;
• and wherein the amino acid sequence from Table 8 or Table 9 and the L protein are co-expressed.