Artificial Sputum and Respiratory Epithelial Model for Bacterial Biofilms in the Respiratory Tract
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsJennifer M. Bomberger, Ananya Kapur
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Abstract
An airway model for culturing microbes such as bacteria Pseudomonas aeruginosa, is provided, comprising culturing the microbes in synthetic sputum media on an air-liquid interface culture of airway epithelial cells. The bacteria may be Pseudomonas aeruginosa, and the epithelial cells may be from a cystic fibrosis (CF) patient and/or comprise a mutation in CFTR found in CF patients.
Claims20
A method of modeling a microbe biofilm, comprising:
- culturing a bacterium or a sputum sample comprising a microbe in a first synthetic sputum media to produce a microbe culture;
- and depositing and culturing the microbe culture in a second synthetic sputum media onto an air liquid interface culture of differentiated airway epithelial cells.
A cell culture device comprising an air-liquid interface culture of differentiated airway epithelial cells and synthetic sputum medium comprising a bacteria or sputum sample deposited on the airway epithelial cells.
A kit comprising an air liquid interface culture of airway epithelial cells and synthetic sputum medium in a container separate from the cell culture device.
Dependencies are inferred from the claim text.