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US 2024/0140992 A1Published application
Hexadepsipeptide compounds and methods of using the same
AssigneeLodo Therapeutics Corporation
InventorsMaria Diarey TIANERO-MCINTOSH, Arlene Atup SY-CORDERO, Bradley Morgan HOVER, Steven L. COLLETTI, Gayatri GOLLAPUDI
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Abstract
The present disclosure provides compounds of Formula (I): or a pharmaceutically acceptable salt, solvate, or tautomer thereof, and uses of the same in treating a disease or disorder (e.g., cancer or fibrosis).
Claims79
A compound of Formula (I):
A method of making a polynucleotide comprising a modified biosynthetic gene cluster comprising:
- a. providing a first E. coli host cell comprising a first vector comprising a sequence of an unmodified biosynthetic gene cluster comprising a target sequence;
- b. introducing the first vector into a Streptomyces host cell by conjugation;
- c. providing a second E. coli host cell comprising a second vector comprising:
- i. a sequence of at least one gNA specific to the target sequence operably linked to a promoter,
- ii. a sequence encoding a Cas9 endonuclease;
- and
- iii. a sequence encoding a donor template;
- and
- d. introducing the second vector into a Streptomyces host cell by conjugation;
- whereby introducing the second vector into the Streptomyces host cell produces a double strand break in the target sequence and introduction of a donor template sequence, thereby generating a Streptomyces host cell comprising a modified biosynthetic gene cluster.
7 more elements
Dependencies are inferred from the claim text.