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US 2024/0139724 A1Published application
Devices and methods for manipulation and concentration of particles and large molecules in a disposable filter tip
AssigneeInnovaPrep LLC
InventorsAndrew E. Page, David Goad, Steven D. Graham
Life of this patent
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Abstract
Highly efficient and rapid filtration-based concentration devices, systems and methods are disclosed with a rotary distribution valve connected to a syringe pump which maintains control and flow of various fluids to a sample through a retentate port.
Claims20
A device, comprising:
- a housing having a top end and a bottom end;
- a filter enclosed within the housing, the filter being vertically oriented and spanning a length of the housing, the filter having a retentate surface and an opposing permeate surface;
- a retentate port positioned adjacent the top end of the housing;
- a permeate port positioned adjacent the top end of the housing;
- a sample port positioned adjacent the bottom end of the housing;
- a rotary distribution valve connected to the retentate port;
- a syringe pump connected to the rotary distribution valve, wherein the syringe pump is adapted to push a volume of fluid that is equivalent to a known internal volume of a retentate portion of the tip;
- and
- a source of addition fluid, a source of blocking fluid, a source of wash fluid;
- and a source of lysis fluid;
- wherein all sources of fluid are connected to separate and distinct ports on the rotary distribution valve.
8 more elements
A method, comprising:
- inserting a retentate port and permeate port of a housing with an enclosed filter into a port in a sample processing device;
- placing a bottom sample port positioned adjacent a bottom end of the housing into a liquid sample;
- drawing a sample using a permeate pump, attached to a housing permeate port, through the filter in the housing, capturing particles on a filter surface;
- selecting one or more of a sample addition fluid, a blocking fluid, a wash fluid, and a lysis fluid, and pushing a volume of fluid into the filter housing before, during, and after processing the sample using a syringe pump and connected rotary distribution valve;
- after processing the sample, pushing a volume of elution fluid through the filter housing to recover particles from the filter surface.
2 more elements
Dependencies are inferred from the claim text.