Multi-component surface chemistry to eliminate retention loss in reversed-phase liquid chromatography
AssigneeWaters Technologies Corporation
InventorFabrice Gritti
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Abstract
Provided herein is a multi-component chromatographic material and use thereof for reversed-phase liquid chromatography. The multi-component chromatographic materials provided herein comprise a chromatographic core having an exterior surface; and at least two different hydrophobic ligands covalently bound to the exterior surface with a total surface coverage less than 2.0 μmol/m2. The multi-component chromatographic materials of the present technology are beneficial for reversed-phase liquid chromatography using highly aqueous mobile phases. For example, chromatographic materials described herein allow mitigating or preventing significant retention loss of reversed-phase liquid chromatography columns after flow interruption.
Claims20
A multi-component chromatographic material comprising:
- a chromatographic core having an exterior surface;
- and
- at least two different hydrophobic ligands covalently bound to the exterior surface, wherein the total surface coverage of the at least two different hydrophobic ligands is less than 2.0 μmol/m2, wherein at least two different hydrophobic ligands comprise a first hydrophobic ligand selected from C4 to C30 phenyl alkyl moiety, and a second hydrophobic ligand selected from C4 to C30 alkyl moiety.
A multi-component chromatographic material comprising:
- a chromatographic core having an exterior surface;
- and
- at least two different hydrophobic ligands covalently bound to the exterior surface, wherein the total surface coverage of the at least two different hydrophobic ligands is less than 2.0 μmol/m2, wherein the first hydrophobic ligand is C18 alkyl moiety and the second hydrophobic ligand is C8 alkyl moiety and the molar ratio of the first hydrophobic ligand to the second hydrophobic ligand is from about 2.0:1.0 to about 1.0:2.0.
A multi-component chromatographic material comprising:
- a chromatographic core having an exterior surface;
- and
- at least two different hydrophobic ligands covalently bound to the exterior surface, wherein the total surface coverage of the at least two different hydrophobic ligands is less than 2.0 μmol/m2, wherein at least two different hydrophobic ligands comprise a first hydrophobic ligand selected from C23 to C42 alkyl moiety, and a second hydrophobic ligand selected from C23 to C42 alkyl moiety, wherein the number of carbon atoms of the second hydrophobic ligand is different than the number of carbon atoms of the first hydrophobic ligand.
Dependencies are inferred from the claim text.