Methods and compositions for detecting and producing porcine morbillivirus and vaccines thereof
AssigneeIowa State University Research Foundation, Inc.
InventorsGanwu Li, Bailey Arruda, Huigang Shen, Ying Zheng
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Abstract
Disclosed herein are methods for detecting and producing PoMV. Further, disclosed herein are immunogenic and/or vaccine compositions and methods for treating or preventing PoMV. The compositions and methods include immunogenic portions of PoMV including entry proteins. In at least particular cases, a mutated version of a portion of the PoMV is utilized, such as a deglycosylated, or amino acid substituted mutant of the spike protein.
Claims27
An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group of:
- a nucleotide sequence comprising a genome sequence of porcine morbillivirus of SEQ ID NO:
- 4;
- a nucleotide sequence which encodes at least 5 contiguous nucleotides SEQ ID NO:
- 45, 48, or 49;
- a nucleotide sequence having at least 90% sequence identity to a SEQ ID NO:
- 4;
- and
- a nucleotide sequence encoding the amino acid sequence of porcine morbillivirus nucleocapsid protein, phosphoprotein, matrix protein, fusion protein, hemagglutinin protein, and/or large protein.
A cell comprising the vector of claim 4.
An immunotherapeutic comprising a porcine morbillivirus epitope.
A method of producing antibodies against porcine morbillivirus, comprising:
- administering to a population of B cells an epitope of porcine morbillivirus;
- allowing antibody production;
- and
- harvesting the produced antibodies.
A method of detecting porcine morbillivirus or a nucleic acid or protein thereof in a sample, comprising:
- obtaining a sample from a subject;
- adding a molecule capable of binding to the porcine morbillivirus or a nucleic acid or protein thereof;
- and
- detecting the binding of the molecule to the porcine morbillivirus or a nucleic acid or protein thereof.
A method of detecting porcine morbillivirus (PoMV) in a sample, the method comprising:
- contacting the sample with a PoMV polypeptide under conditions that allow the formation of an antigen-antibody complex between said PoMV polypeptide and a PoMV antibody present in the sample, wherein the PoMV polypeptide comprises a fragment of at least 5 amino acids and wherein the PoMV peptide comprises at least 90% identity to the amino acid sequence of SEQ ID NOs:
- 45, 48, and/or 49;
- and detecting the antigen-antibody complex formed.
An amino acid sequence encoding porcine morbillivirus (PoMV) nucleocapsid protein, phosphoprotein, matrix protein, fusion protein, hemagglutinin protein, and/or large protein.
Dependencies are inferred from the claim text.