Homogenous enzyme linked immunoassay
AssigneeIDEXX Laboratories, Inc.
InventorsJennifer Gagner, Dheeraj Khare, Daniel Wayne Patch, Evan Michael Peck, James Robert Salter, Christopher Peter Turmel
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Abstract
The present disclosure relates to methods for determining the presence of or the amount of an analyte in a sample and to a slide for use in the method.
Claims38
A slide for determining the presence of or the amount of an analyte in a sample comprising:
- (i) a first layer comprising a binding partner-enzyme conjugate dispersed in a first polymer, wherein the binding partner-enzyme conjugate is specific for the analyte;
- (ii) a second layer comprising solid particles that have analyte bound to their surface dispersed in a second polymer, and
- (iii) a third layer comprising a substrate for the enzyme dispersed in a third polymer, wherein interaction of the substrate and the enzyme produces a detectable signal.
A slide for determining the presence of or the amount of an analyte in a sample comprising:
- (i) a first layer comprising a binding partner-enzyme conjugate attached to the surface of particles dispersed in a first polymer, wherein the binding partner-enzyme conjugate is specific for the analyte;
- (ii) a second layer comprising a substrate for the enzyme dispersed in a second polymer, wherein interaction of the substrate and the enzyme produces a detectable signal.
A method for determining the presence of or the amount of an analyte in a sample comprising:
- (i) providing a sample suspected of containing an analyte;
- (ii) contacting the sample with a binding partner-enzyme conjugate attached to the surface of particles to provide a mixture of comprising the binding partner-enzyme conjugate attached to the surface of particles and the sample, wherein analyte in the mixture can displace the binding partner-enzyme conjugate from the surface of the particles,
- (iii) separating the particles from the mixture to provide a particle free mixture;
- (iv) contacting the particle free mixture with a polymer comprising a substrate for the enzyme dispersed in the polymer, wherein interaction of the substrate and the enzyme produces a detectable signal.
A dry slide for determining the presence of or the amount of an analyte in a sample comprising dried laccase.
A dry slide for determining the presence of or the amount of an analyte in a sample comprising TMB and succinic acid.
Dependencies are inferred from the claim text.