Method of detecting multiple forms of an analyte

A method of determining whether any of multiple forms of a nucleic acid analyte are present in a sample, the method comprising the steps of:

• (a) providing a sample to an analyzer;
• (b) producing a purified form of the sample by exposing the sample to reagents and conditions adapted to isolate and purify multiple forms of a nucleic acid analyte;
• (c) dissolving an amplification reagent with a first solvent, wherein the amplification reagent contains oligonucleotides sufficient to amplify and detect a first region of a first form of the analyte, wherein the first solvent contains one or more oligonucleotides which, in combination with the oligonucleotides of the amplification reagent, are sufficient to amplify and detect a second region of a second form of the analyte, wherein the one or more oligonucleotides of the first solvent are insufficient to amplify and detect the first or second form of the analyte, and wherein the first and second regions each comprise a different nucleotide base sequence;
• (d) contacting the purified form of the sample with the dissolved amplification reagent, thereby forming an amplification reaction mixture;
• (e) exposing the amplification reaction mixture to temperature conditions sufficient for amplifying the first and second regions of the first and second forms of the analyte, respectively;
• and
• (f) determining whether at least one of the first and second forms of the analyte is present in the sample.