Enzymatic method for preparation of gdp-fucose
AssigneeMax-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.
InventorsReza Mahour, Thomas F.T. Rexer
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Abstract
The present invention relates to an enzyme-catalyzed process for producing GDP-fucose from low-cost substrates guanosine and L-fucose or guanosine and D-Mannose in a single reaction mixture. Said process can be operated (semi)continuously or in batch mode. Further, said process can be adapted to produce fucosylated molecules and biomolecules including glycans, such as human milk oligosaccharides, proteins, peptides, glycoproteins or glycopeptides.
Claims17
-19. (canceled)
A method for producing guanosine 5′-diphospho-β-L-fucose from guanosine and D-mannose comprising the following steps:
- A) providing a solution comprising
- (i) guanosine and D-mannose represented by the following formulae
- (ii) polyphosphate, adenosine triphosphate, a co-solvent for solubilizing guanosine and NADPH;
- and
- providing a set of enzymes comprising a guanosine kinase, a polyphosphate kinase, a mannose-1-phosphate guanylyltransferase, a GDP-mannose-4,6-dehydratase, a GDP-L-fucose-synthase and either (a) a glucokinase and a phosphomannomutase, or (b) an N-acetylhexosamine-1-kinase.
- B) producing guanosine 5′-diphospho-β-L-fucose from guanosine and D-mannose in the presence of the set of enzymes, polyphosphate, adenosine triphosphate, NADPH and the co-solvent.
Dependencies are inferred from the claim text.