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US 2024/0124902 A1Published application
Enzyme-expressing yeast for ethanol production
AssigneeNovozymes A/S
InventorsMichael Glenn Catlett, Monica Tassone, Paul Vincent Harris, Robert Lyle Osborne, Shiro Fukuyama, Tomoko Matsul, Ryoko Kataoka
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Abstract
Described herein are recombinant fermenting organisms having a heterologous polynucleotide encoding an alpha-amylase and/or a heterologous polynucleotide encoding a trehalase. Also described are processes for producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant fermenting organisms.
Claims21
1-23. (canceled)
24:
- A method of producing a fermentation product from a starch-containing or cellulosic-containing material comprising:
- (a) saccharifying the starch-containing or cellulosic-containing material;
- and
- (b) fermenting the saccharified material of step (a) with a fermenting organism;
- wherein the fermenting organism comprises a heterologous polynucleotide encoding an alpha-amylase or a heterologous polynucleotide encoding a trehalase.
2 more elements
25:
- The method of claim 24, wherein the alpha-amylase has a mature polypeptide sequence with at least 80% sequence identity to the amino acid sequence of any one of SEQ ID NOs:
- 76-101, 121-174 and 231.
26:
- The method of claim 24, wherein the alpha-amylase has a mature polypeptide sequence comprising or consisting of the amino acid sequence of any one of SEQ ID NOs:
- 76-101, 121-174 and 231.
27:
- The method of claim 24, wherein the trehalase has mature polypeptide sequence with at least 80% sequence identity to the amino acid sequence of any one of SEQ ID NOs:
- 175-226.
28:
- The method of claim 24, wherein the trehalase has a mature polypeptide sequence comprising or consisting of the amino acid sequence of any one of SEQ ID NOs:
- 175-226.
29:
- The method of claim 24, wherein saccharification of step (a) occurs on a starch-containing material.
30:
- The method of claim 29, comprising liquefying the starch-containing material by contacting the material with an alpha-amylase prior to saccharification.
31:
- The method of claim 30, wherein liquefying the starch-containing material and/or saccharifying the starch-containing material is conducted in presence of exogenously added protease.
32:
- The method of claim 24, wherein fermentation and saccharification are performed simultaneously in a simultaneous saccharification and fermentation (SSF).
33:
- The method of claim 24, comprising recovering the fermentation product from the from the fermentation.
34:
- The method of claim 24, wherein the fermentation product is ethanol.
35:
- The method of claim 24, wherein the fermenting organism comprises a heterologous polynucleotide encoding a glucoamylase or a protease.
36:
- The method of claim 24, wherein the fermenting organism is a Saccharomyces cerevisiae cell.
37:
- A recombinant yeast cell comprising a heterologous polynucleotide encoding an alpha-amylase or a heterologous polynucleotide encoding a trehalase.
38:
- The recombinant yeast cell of claim 37, wherein the alpha-amylase has a mature polypeptide sequence with at least 80% sequence identity to the amino acid sequence of any one of SEQ ID NOs:
- 76-101, 121-174 and 231.
39:
- The recombinant yeast cell of claim 37, wherein the alpha-amylase has a mature polypeptide sequence comprising or consisting of the amino acid sequence of any one of SEQ ID NOs:
- 76-101, 121-174 and 231.
40:
- The recombinant yeast cell of claim 37, wherein the trehalase has mature polypeptide sequence with at least 80% sequence identity to the amino acid sequence of any one of SEQ ID NOs:
- 175-226.
41:
- The recombinant yeast cell of claim 37, wherein the trehalase has a mature polypeptide sequence comprising or consisting of the amino acid sequence of any one of SEQ ID NOs:
- 175-226
42:
- The recombinant yeast cell of claim 37, wherein the fermenting organism comprises a heterologous polynucleotide encoding a glucoamylase or a protease.
43:
- The recombinant yeast of claim 37, wherein the cell is a Saccharomyces cerevisiae cell.