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US 2024/0123446 A1Published application
Single cell sensing and selection
AssigneeHewlett-Packard Development Company, L.P.
InventorsAlexander Govyadinov, Viktor Shkolnikov
Life of this patent
Estimating the term…
Abstract
A microfluidic system may comprise a dispense head with multiple dispensers, each dispensing a different cell type, such as single pairs of individual target cells and individual sensor cells. Interaction between the cells may be observed based on, for example, fluorescence. Individual target cells may then be selected, based on observations, for use or for further investigation. As an example, target cells may be B-cells, and enhanced selection of B-cells aids more direct antibody discovery.
Claims20
A microfluidic system comprising:
- a dispense head comprising:
- a first dispenser to dispense a first biological cell type and a second dispenser to dispense a second biological cell type;
- a first dispenser channel to load cells into a first cell staging chamber to be dispensed by the first dispenser, and a second dispenser channel to load cells into a second cell staging chamber to be dispensed by the second dispenser;
- and
- one or more sensing circuits to detect single cells in the first dispenser channel and in the second dispenser channel;
- and
- a controller to use the dispense head to dispense, in each individual well of a multiwell plate, an individual first cell from the first cell staging chamber and an individual second cell from the second dispenser cell staging chamber.
4 more elements
A method comprising:
- using a dispense head to dispense, in each individual well of a multiwell plate, an individual first cell from a first cell staging chamber and an individual second cell from a second dispenser cell staging chamber such that the individual first cell and the individual second cell interact in the individual well, the dispense head comprising:
- a first dispenser to dispense a first biological cell type and a second dispenser to dispense a second biological cell type;
- a first dispenser channel to load cells into the first cell staging chamber to be dispensed by the first dispenser, and a second dispenser channel to load cells into the second cell staging chamber to be dispensed by the second dispenser;
- and
- one or more sensing circuits to detect single cells in the first dispenser channel and in the second dispenser channel.
2 more elements
A microfluidic system comprising:
- a set of dispense heads, each dispense head comprising:
- a first dispenser to dispense a first biological cell type and a second dispenser to dispense a second biological cell type;
- a first dispenser channel to load cells into a first cell staging chamber ready to be dispensed by the first dispenser, and a second dispenser channel to load cells into a second cell staging chamber ready to be dispensed by the second dispenser;
- and
- one or more sensing circuits to detect single cells in the first dispenser channel and the second dispenser channel to enable one cell at a time to be dispensed by the first dispenser and the second dispenser, respectively;
- wherein the set of dispense heads have a staggered arrangement in which each dispense head in a first subset of the set of dispense heads extends farther out than each dispense head in a second subset of the set of dispense heads.
3 more elements
Dependencies are inferred from the claim text.