High amylose wheat- ii
AssigneeArista Cereal Technologies Pty Limited
InventorsBernard Duperrier, Pierre Georges Louis Berbezy, Elisabeth Marie-Anne Ida Chanliaud, Ahmed Regina, Matthew Kennedy Morell
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Abstract
Wheat grain (Triticum aestivum) comprising an embryo and starch, wherein the embryo comprises two identical alleles of an SBEIIa-A gene, two identical alleles of an SBEIIa-B gene and two identical alleles of an SBEIIa-D gene, wherein each of the SBEIIa genes gives rise to an amount of protein (w/w) or a protein having SBEIIa activity which is lower than the corresponding wild-type gene, and at least one of said genes comprises a point mutation, wherein the starch comprises amylose such that the grain has an amylose content of at least 50% (w/w) as a proportion of the extractable starch of the grain.
Claims10
1-49. (canceled)
Durum wheat grain comprising an embryo and starch, wherein
- (a) the embryo comprises two identical null alleles of an SBEIIa-A gene and two identical null alleles of an SBEIIa-B gene, wherein either the two identical null alleles of the SBEIIa-A gene, or the two identical null alleles of the SBEIIa-B gene, comprise point mutations or deletions of 1-30 basepairs;
- (b) SBEIIa protein is undetectable in the wheat grain;
- (c) the starch comprises amylose such that the grain has an amylose content of between 50% and 90% (w/w) as a proportion of the extractable starch of the grain;
- and
- (d) the wheat grain has a germination rate of about 70% to about 100% relative to the germination rate of a wild-type wheat grain.
A process of producing a durum wheat plant that produces grain comprising an amylose content of at least 50% (w/w), as a proportion of the total starch in the grain, the process comprising:
- (i) crossing two parental durum wheat plants each comprising a point mutation or deletion of 1-30 basepairs in a SBEIIa gene selected from the group consisting of SBEIIa-A and SBEIIa-B, wherein the two parental wheat plants together comprise null mutations in each of the SBEIIa-A and SBEIIa-B genes,
- (ii) screening plants or grain obtained from the cross, or progeny plants or grain obtained therefrom, by analysing DNA, RNA, protein, starch granules or starch from the plants or grain, and
- (iii) selecting a fertile plant that lacks SBEIIa protein.
Dependencies are inferred from the claim text.