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US 2024/0117331 A1Published application
Polypeptides having pectinase activity, polynucleotides encoding same, and uses thereof
AssigneeNovozymes A/S
InventorsGeoffrey Moxley, Mercy Wada, Monica Tassone, Mary Ann Stringer, Hong Zhi Huang, Nikolaj Spodsberg, Tianqi Sun, Sara Maria Landvik, James Lavigne, Cui Liu
Life of this patent
Estimating the term…
Abstract
The present invention relates to isolated polypeptides having pectinase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Claims22
An isolated or purified polypeptide having pectinase activity, wherein the polypeptide having pectinase activity is selected from the group consisting of:
- (a)
- (i) a polypeptide having at least 99.6% identity, at least 99.7% identity, at least 99.8% or at least 99.9% identity to the mature polypeptide of SEQ ID NO:
- 5 and having pectinase activity;
- (ii) a polypeptide having at least 99.6% identity, at least 99.7% identity, at least 99.8% or at least 99.9% identity to the mature polypeptide of SEQ ID NO:
- 6, which has pectinase activity;
- (iii) a polypeptide having at least 99.6% identity, at least 99.7% identity, at least 99.8% or at least 99.9% identity to a mature polypeptide of SEQ ID NO:
- 5, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 4, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 99.6% identity, at least 99.7% identity, at least 99.8% or at least 99.9% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 4, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 5 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 5, which has pectinase activity;
- and
- (vii) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- (b)
- (i) a polypeptide having at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide of SEQ ID NO:
- 11, which has pectinase activity;
- (ii) a polypeptide having at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide of SEQ ID NO:
- 12, which has pectinase activity;
- (iii) a polypeptide having at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 11, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 10 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 10 or the cDNA sequence thereof, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 11 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 11, pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity.
- (c)
- (i) polypeptide having at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 14, which has pectinase activity;
- (ii) a polypeptide having at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 15, which has pectinase activity;
- (iii) a polypeptide having at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 14, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 13 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 13 or the cDNA sequence thereof, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 14 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 14, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- (d)
- (i) polypeptide having at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 17, which has pectinase activity;
- (ii) a polypeptide having at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 18, which has pectinase activity;
- (iii) a polypeptide at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 17, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 16 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 16 or the cDNA sequence thereof, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 17 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 17, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- (e)
- (i) polypeptide having at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 20, which has pectinase activity;
- (ii) a polypeptide having at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 21, which has pectinase activity;
- (iii) a polypeptide having at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 19, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 19 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 19 or the cDNA sequence thereof, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 20 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 20, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- (f)
- (i) polypeptide having at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 38, which has pectinase activity;
- (ii) a polypeptide having at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 39, which has pectinase activity;
- (iii) a polypeptide having at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 38, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 37 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 37 or the cDNA sequence thereof, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 38 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 38, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- (g)
- (i) polypeptide having at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 41, which has pectinase activity;
- (ii) a polypeptide having at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 42, which has pectinase activity;
- (iii) a polypeptide having at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 41, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 40 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 40 or the cDNA sequence thereof, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 41 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 41, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- (h)
- (i) polypeptide having at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 44, which has pectinase activity;
- (ii) a polypeptide having at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 45, which has pectinase activity;
- (iii) a polypeptide having at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 44, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 43 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 43 or the cDNA sequence thereof, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 44 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 44, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- (i)
- (i) polypeptide having at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 47, which has pectinase activity;
- (ii) a polypeptide having at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 48, which has pectinase activity;
- (iii) a polypeptide having at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 47, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 46 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 46 or the cDNA sequence thereof, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 47 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 47, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- (j)
- (i) polypeptide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 53, which has pectinase activity;
- (ii) a polypeptide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 54, which has pectinase activity;
- (iii) a polypeptide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 53, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 52, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 52, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 53 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 53, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- (k)
- (i) polypeptide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 56, which has pectinase activity;
- (ii) a polypeptide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 57, which has pectinase activity;
- (iii) a polypeptide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 56 which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 55 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 55 or the cDNA sequence thereof, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 56 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 56, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- (l)
- (i) polypeptide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 59, which has pectinase activity;
- (ii) a polypeptide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 60, which has pectinase activity;
- (iii) a polypeptide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 59, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 58 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 58 or the cDNA sequence thereof, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 59 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 59, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- (m)
- (i) polypeptide having at least 98% identity, at least 98.5% identity, at least 99% identity, at least 99.5% identity, at least 99.6% identity, at least 99.7% identity, at least 99.8% identity, or at least 99.9% identity to SEQ ID NO:
- 62, which has pectinase activity;
- (ii) a polypeptide having at least 98% identity, at least 98.5% identity, at least 99% identity, at least 99.5% identity, at least 99.6% identity, at least 99.7% identity, at least 99.8% identity, or at least 99.9% identity to SEQ ID NO:
- 63, which has pectinase activity;
- (iii) a polypeptide having at least 98% identity, at least 98.5% identity, at least 99% identity, at least 99.5% identity, at least 99.6% identity, at least 99.7% identity, at least 99.8% identity, or at least 99.9% identity to a mature polypeptide of SEQ ID NO:
- 62, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 61 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 98% identity, at least 98.5% identity, at least 99% identity, at least 99.5% identity, at least 99.6% identity, at least 99.7% identity, at least 99.8% identity, or at least 99.9% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 61 or the cDNA sequence thereof, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 62 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 62, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- (n)
- (i) polypeptide having at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 68, which has pectinase activity;
- (ii) a polypeptide having at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 69, which has pectinase activity;
- (iii) a polypeptide having at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 68, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 67 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 67 or the cDNA sequence thereof, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 68 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 68, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has having pectinase activity;
- (o)
- (i) polypeptide having at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 71, which has pectinase activity;
- (ii) a polypeptide having at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 72, which has pectinase activity;
- (iii) a polypeptide having at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 71, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 70 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 70 or the cDNA sequence thereof, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 71 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 71, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- (p)
- (i) polypeptide having at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 74, which has pectinase activity;
- (ii) at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 75, which has pectinase activity;
- (iii) a polypeptide having at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 74, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 73 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 73 or the cDNA sequence thereof, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 74 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 74, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- (q)
- (i) polypeptide having at least 60% identity, at least 65% identity, at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 83, which has pectinase activity;
- (ii) a polypeptide having at least 60% identity, at least 65% identity, at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 84, which has pectinase activity;
- (iii) a polypeptide having at least 60% identity, at least 65% identity, at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 83, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 82 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 60% identity, at least 65% identity, at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 82 or the cDNA sequence thereof, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 83 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 83, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- (r)
- (i) polypeptide having at least 98.6% identity, at least 98.7% identity, at least 98.8% identity, at least 98.9% identity, at least 99% identity, at least 99.1% identity, at least 99.2% identity, at least 99.3% identity, at least 99.4% identity, at least 99.5% identity, at least 99.6% identity, at least 99.7% identity, at least 99.8% identity, or at least 99.9% identity to SEQ ID NO:
- 89, which has pectinase activity;
- (ii) a polypeptide having at least 98.6% identity, at least 98.7% identity, at least 98.8% identity, at least 98.9% identity, at least 99% identity, at least 99.1% identity, at least 99.2% identity, at least 99.3% identity, at least 99.4% identity, at least 99.5% identity, at least 99.6% identity, at least 99.7% identity, at least 99.8% identity, or at least 99.9% identity to SEQ ID NO:
- 90, which has pectinase activity;
- (iii) a polypeptide having at least 98.6% identity, at least 98.7% identity, at least 98.8% identity, at least 98.9% identity, at least 99% identity, at least 99.1% identity, at least 99.2% identity, at least 99.3% identity, at least 99.4% identity, at least 99.5% identity, at least 99.6% identity, at least 99.7% identity, at least 99.8% identity, or at least 99.9% identity to a mature polypeptide of SEQ ID NO:
- 89, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 88, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 98.6% identity, at least 98.7% identity, at least 98.8% identity, at least 98.9% identity, at least 99% identity, at least 99.1% identity, at least 99.2% identity, at least 99.3% identity, at least 99.4% identity, at least 99.5% identity, at least 99.6% identity, at least 99.7% identity, at least 99.8% identity, or at least 99.9% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 88, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 89 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 89, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- (s)
- (i) polypeptide having at least 60% identity, at least 65% identity, at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 92, which has pectinase activity;
- (ii) a polypeptide having at least 60% identity, at least 65% identity, at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 93, which has pectinase activity;
- (iii) a polypeptide having at least 60% identity, at least 65% identity, at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 92, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 91 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 60% identity, at least 65% identity, at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 91 or the cDNA sequence thereof, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 92 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 92, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- (t)
- (i) polypeptide having at least 60% identity, at least 65% identity, at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 137, which has pectinase activity;
- (ii) a polypeptide having at least 60% identity, at least 65% identity, at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 138, which has pectinase activity;
- (iii) a polypeptide having at least 60% identity, at least 65% identity, at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 137, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 136 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 60% identity, at least 65% identity, at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 136 or the cDNA sequence thereof, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 137 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 137, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- (u)
- (i) polypeptide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 140, which has pectinase activity;
- (ii) a polypeptide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 141, which has pectinase activity;
- (iii) a polypeptide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 140, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 139 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 139 or the cDNA sequence thereof, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 140 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 140, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- (v)
- (i) polypeptide having at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 143, which has pectinase activity;
- (ii) a polypeptide having at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 144, which has pectinase activity;
- (iii) a polypeptide having at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 143, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 142 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 142 or the cDNA sequence thereof, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 143 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 143, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity;
- and
- (w)
- (i) polypeptide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 146, which has pectinase activity;
- (ii) a polypeptide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to SEQ ID NO:
- 147, which has pectinase activity;
- (iii) a polypeptide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to a mature polypeptide of SEQ ID NO:
- 146, which has pectinase activity;
- (iv) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, or high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 145 or the cDNA sequence thereof, which has pectinase activity;
- (v) a polypeptide encoded by a polynucleotide having at least 70% identity, at least 71% identity, at least 72% identity, at least 73% identity, at least 74% identity, at least 75% identity, at least 76% identity, at least 77% identity, at least 78% identity, at least 79% identity, at least 80% identity, at least 81% identity, at least 82% identity, at least 83% identity, at least 84% identity, at least 85% identity, at least 86% identity, at least 87% identity, at least 88% identity, at least 89% identity, at least 90% identity, at least 91% identity, at least 92% identity, at least 93% identity, at least 94% identity, at least 95% identity, at least 96% identity, at least 97% identity, at least 98% identity, or at least 99% identity to the mature polypeptide coding sequence of SEQ ID NO:
- 145, which has pectinase activity;
- (vi) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 146 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 137, which has pectinase activity;
- and
- (vi) a fragment of the polypeptide of (i), (ii), (iii), (iv), (v) or (vi) that has pectinase activity.
366 more elements
A method of producing a polypeptide having pectinase activity, comprising cultivating the recombinant host cell of claim 13 under conditions conducive for production of the polypeptide, and optionally recovering the polypeptide.
A process for producing fermentation products from starch-containing material comprising:
- i) saccharifying the starch-containing material using a carbohydrate-source generating enzyme at a temperature below the initial gelatinization temperature;
- ii) fermenting using a fermenting organism;
- wherein at least one polypeptide having pectinase activity is present or added during saccharifying step i) or fermenting step ii), and wherein the at least one polypeptide having pectinase activity is selected from the group consisting of a polygalacturonase from the GH28 family, a rhamnogalacturonase, from the GH28 family, a pectin methyl esterase from the CE8 family, a pectin lyase from the PL1 family, a pectin acetyl esterase from the CE12 family or CE16 family, a rhamnogalacturonan lyase from the PL4 family, a beta-galactanase from the GH53 family, and combinations thereof.
A process for producing fermentation products from starch-containing material comprising the steps of:
- i) liquefying the starch-containing material at a temperature above the initial gelatinization temperature using an alpha-amylase;
- ii) saccharifying using a carbohydrate-source generating enzyme;
- iii) fermenting using a fermenting organism;
- wherein at least one polypeptide having pectinase activity is present or added during saccharifying step i) or fermenting step ii), and wherein the at least one polypeptide having pectinase activity is selected from the group consisting of a polygalacturonase from the GH28 family, a rhamnogalacturonase, from the GH28 family, a pectin methyl esterase from the CE8 family, a pectin lyase from the PL1 family, a pectin acetyl esterase from the CE12 family or CE16 family, a rhamnogalacturonan lyase from the PL4 family, a beta-galactanase from the GH53 family, and combinations thereof.
1 more element
(canceled)
(canceled)
(canceled)
Dependencies are inferred from the claim text.