Surface Modified Red Blood Cells And Methods Of Generating The Same
AssigneesNational University of Singapore, City University of Hong Kong
InventorsThi Nguyet Minh LE, Migara Kavishka JAYASINGHE, Boya PENG, Jiahai SHI
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Abstract
The present invention relates to methods modifying cell surface markers of red blood cells (RBCs) and uses of the same. In particular, the method comprises contacting an RBC with a peptide in the presence of a ligase, under suitable conditions and for sufficient time to allow ligation of the peptide to the RBC to form an RBC-peptide conjugate. In one embodiment, the ligase is OaAEPI ligase. The RBC-peptide conjugate may be further contacted with an effector molecule under suitable conditions and for sufficient time for conjugation of the effector molecule to the RBC-peptide to form an RBC-peptide-effector molecule conjugate.
Claims30
A method comprising:
- (a) contacting a Red Blood Cell (RBC) with a peptide in the presence of a ligase, under suitable conditions and for sufficient time to allow ligation of the peptide to the RBC to form an RBC-peptide conjugate;
- wherein the peptide comprises a C-terminal ligase recognition sequence;
- and optionally,
- wherein the method further comprises a step of washing the RBC-peptide conjugate.
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A modified RBC comprising, on its exterior surface, a peptide, wherein the peptide is conjugated to a native red blood cell protein.
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Dependencies are inferred from the claim text.