Orthopoxvirus serology assays
OwnerGeorge SIGAL, Leonid DZANTIEV +2inventors
InventorsGeorge SIGAL, Leonid DZANTIEV, Priscilla KRAI, Teri-Tu B. NGO
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Abstract
The invention relates to methods and kits for detecting one or more antibody biomarkers in a sample, wherein the antibody biomarker specifically binds a viral antigen. In embodiments, the viral antigen is a monkeypox virus (MPXV) antigen, a vaccinia virus (VACV) antigen, or combination thereof.
Claims32
A kit for detecting one or more antibody biomarkers of interest in a sample, the kit comprising, in one or more vials, containers, or compartments:
- a. a surface comprising a panel of antigens immobilized on one or more binding domains, wherein each binding domain comprises an antigen of the panel of antigens immobilized thereon, wherein the panel of antigens comprises a monkeypox virus (MPXV) protein, a vaccinia virus (VACV) protein, or combination thereof;
- and
- b. one or more detection reagents, wherein each detection reagent comprises a detection antibody, a detection antigen, or a detection competitor.
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A method of detecting one or more antibody biomarkers of interest in a sample, comprising:
- (a) forming one or more binding complexes, wherein each binding complex comprises an antigen;
- an antibody biomarker that binds to the antigen;
- and a detection reagent, wherein the antigen comprises a monkeypox virus (MPXV) protein, a vaccinia virus (VACV) protein, or combination thereof;
- and
- (b) detecting the one or more binding complexes, thereby detecting the one or more antibody biomarkers in the sample.
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A method of detecting one or more antibody biomarkers of interest in a sample, comprising:
- (a) contacting the sample with a surface comprising a panel of antigens immobilized on one or more binding domains, wherein each binding domain comprises an antigen of the panel of antigens immobilized thereon, wherein the panel of antigens comprises a monkeypox virus (MPXV) protein, a vaccinia virus (VACV) protein, or combination thereof;
- (b) forming a binding complex in each binding domain, wherein each binding complex comprises the antigen and an antibody biomarker that binds to the antigen;
- (c) contacting the binding complex in each binding domain with a detection reagent;
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- (d) detecting the binding complexes on the surface, thereby detecting the one or more antibody biomarkers in the sample.
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Dependencies are inferred from the claim text.