Genetically modified organisms for the production of steroid derivatives
AssigneeSandhill One, LLC
InventorsJAYACHANDRA P. REDDY, J. GREGORY REID, RICHARD REUMERMAN, BERNHARD J. PAUL, URSULA SCHELL
Life of this patent
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Abstract
Provided are biosynthetic processes for producing sterol derivatives, and to non-naturally occurring organisms capable of producing sterol derivatives. More specifically, genetically modified non-naturally occurring organisms for producing KCEA, KCDA, and related compounds, from cholesterol, β-sitosterol, campesterol and their analogs, are provided.
Claims30
1) A non-naturally occurring steroid degrading microbial organism comprising the following enzymatic activities disrupted:
- aerobic 9,10-seco degradation activity and 4-(steroid-17-yl)pentanoyl side chain degradation activity.
2) A non-naturally occurring steroid degrading microbial organism comprising the following enzymatic activities disrupted:
- steroid 9-alpha-hydroxylation activity and 4-(steroid-17-yl)pentanoyl side chain degradation activity.
4) A non-naturally occurring steroid degrading microbial organism characterized by the following isoenzymes disrupted:
- a) one or more 3-ketosteroid-9-alpha-hydroxylase isoenzymes disrupted at an oxygenase subunit, a ferredoxin reductase subunit, or a combination thereof;
- and
- b) one or more acyl-CoA dehydrogenase isoenzymes that act on steroid CoA esters having five carbon side chains.
5) A non-naturally occurring steroid degrading microbial organism characterized by the following enzymatic activities disrupted:
- a) one or more 3-ketosteroid-9-alpha-hydroxylase isoenzymes disrupted at an oxygenase subunit, a ferredoxin reductase subunit, or a combination thereof;
- b) one or more acyl-CoA dehydrogenase isoenzymes that act on steroid CoA esters having five carbon side chains;
- and
- c) one or more 3-ketosteroid delta-1 dehydrogenase isoenzymes.
7) The non-naturally occurring microbial organism of any of the foregoing claims derived from the genus Rhodococcus.
8) The non-naturally occurring microbial organism of any of the foregoing claims derived from Rhodococcus jostii RHA1.
9) The non-naturally occurring microbial organism of any of the foregoing claims derived from Rhodococcus sp. DSM 1444 or DSM 1445.
Dependencies are inferred from the claim text.