Enzymes Entrapped in Organopolysiloxane Matrix
OwnerJohn Aikens, LeAnne M. Cabalka Tourtelotteinventors
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Abstract
A stabilized enzyme product comprising composite particles that comprise an enzyme component within a nanoporous organopolysiloxane matrix. The enzyme component is capable of hydrolyzing one or more acyl-homoserine lactones and constitutes about 0.01 wt % to about 40 wt % of the composite particles on a dry weight basis. The composite particles have a density in a range of about 0.02 g/cm3 to about 0.5 g/cm3, an accessible surface area in a range of about 250 m2/g to about 600 m2/g, and fractal three-dimensional structures.
Claims33
A method of stabilizing an enzyme, the method comprising:
- forming a hydrolysis reaction mixture that comprises a siloxane monomer component, an acid component, and a solvent component, wherein:
- the acid component is at an amount sufficient for the hydrolysis reaction mixture to have a pH in a range of about 1 to about 6 and to catalyze the hydrolysis of the siloxane component thereby producing an oligo-siloxane sol that comprises siloxane oligomers;
- the solvent component comprises water at an amount such that a mol ratio of water to silicon of the siloxane monomer component is in a range of about 0.2:1 to about 100:1;
- and
- the siloxane monomer component comprises:
- one or more tetraalkoxysilane monomers having the general formula Si(OR)4 or (OR)3Si(OSi(OR)2)nOSi(OR)3;
- and
- one or more organo-alkoxysiloxane monomers having the general formula Si(OR)4-xR′x;
- wherein:
- n is in the range of 0-100;
- x is in the range of 1 to 3;
- each of the R groups is selected from the group consisting of methyl, ethyl, propyl, isopropyl, and butyl alkyl;
- and
- each R′ is selected from the group consisting of methyl, ethyl, propyl, isopropyl, butyl, octyl, hexadecyl, octadecyl, phenyl, trimethylaminopropyl, aminopropyl, aminobutyl, methylphosphonyl, hydroxymethyl, hydroxypropyl, methoxypropyl, methoxymethyl, acetoxymethyl, acetoxypropyl, acetoxypolyethlyeneoxypropyl, benzyl, carboxymethoxyethyl, epoxyhexyl, glycidoxypropyl, isocyanopropyl, and phenethyl;
- and
- wherein the one or more organo-alkoxysiloxane monomers are at an amount that is greater than 0 mol % and no more than about 30 mol % of the siloxane monomer component on a silicon mol basis;
- removing any non-aqueous solvents from the oligo-siloxane sol to form an aqueous oligo-siloxane sol;
- forming a gelation reaction mixture that comprises the aqueous oligo-siloxane sol, an enzyme component, and a base component and/or a buffer component, wherein the gelation reaction mixture has a pH in a range of about 7 to about 10, wherein the base component and/or the buffer component catalyze the gelation of the aqueous oligosiloxane sol thereby producing a gel that comprises a solid phase and liquid phase, wherein the enzyme component is capable of hydrolyzing one or more acyl-homoserine lactones, wherein the solid phase comprises the enzyme component entrapped in nanoporous organopolysiloxane, and wherein the entrapped enzyme component constitutes about 0.01 wt % to about 40 wt % of the solid phase on a dry weight basis;
- and
- curing the gel to form a solid hydrogel;
- thereby stabilizing the enzyme.
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A stabilized enzyme product comprising composite particles that comprise an enzyme component within a nanoporous organopolysiloxane matrix wherein:
- the enzyme component is capable of hydrolyzing one or more acyl-homoserine lactones;
- the enzyme component constitutes about 0.01 wt % to about 40 wt % of the composite particles on a dry weight basis;
- the composite particles have a density in a range of about 0.02 g/cm3 to about 0.5 g/cm3, an accessible surface area in a range of about 250 m2/g to about 600 m2/g, and fractal three-dimensional structures;
- and
- the nanoporous organopolysiloxane is derived from a siloxane monomer component that comprises:
- one or more tetraalkoxysilane monomers having the general formula Si(OR)4 or (OR)3Si(OSi(OR)2)nOSi(OR)3;
- and
- one or more organo-alkoxysiloxane monomers having the general formula Si(OR)4-x1R′x;
- wherein:
- n is in the range of 0 to 100;
- x is in the range of 1 to 3;
- each of the R groups is selected from the group consisting of methyl, ethyl, propyl, isopropyl, and butyl alkyl;
- and
- each R′ is selected from the group consisting of methyl, ethyl, propyl, isopropyl, butyl, octyl, hexadecyl, octadecyl, phenyl, trimethylaminopropyl, aminopropyl, aminobutyl, methylphosphonyl, hydroxymethal, acetoxymethyl, acetoxypropyl, acetoxypolyethyleneoxypropyl, benzyl, carboxymethoxyethyl, expoxyhexyl, glycidoxypropyl, isocyanatopropyl, and phenethyl;
- and
- wherein the one or more organo-alkoxysiloxane monomers are at an amount that is greater than 0 mol % and no more than about 30 mol % of the siloxane monomer component on a silicon mol basis.
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A method of hydrolyzing one or more acyl-homoserine lactones produced by one or more organisms of a gut microbiome within a gut of an individual, the method comprising administering an effective amount of a stabilized enzyme product to the patient such that the stabilized enzyme product reaches the gut of the patient, and, while in the gut, the enzyme component remains active within the nanoporous organopolysiloxane matrix and hydrolyzes the one or more acyl-homoserine lactones produced by one or more organisms of a gut microbiome within the gut of the patient, wherein the stabilized enzyme product comprises composite particles that comprise an enzyme component within a nanoporous organopolysiloxane matrix wherein:
- the enzyme component is capable of hydrolyzing one or more acyl-homoserine lactones;
- the enzyme component constitutes about 0.01 wt % to about 40 wt % of the composite particles on a dry weight basis;
- the composite particles have a density in a range of about 0.02 q/cm3 to about 0.5 g/cm3, an accessible surface area in a range of about 250 m2/g to about 600 m2/g, and fractal three-dimensional structures;
- and
- the nanoporous organopolysiloxane is derived from a siloxane monomer component that comprises:
- one or more tetraalkoxysilane monomers having the general formula Si(OR)4 or (OR)3Si(OSi(OR)2)nOSi(OR)3;
- and
- one or more organo-alkoxysiloxane monomers having the general formula Si(OR)4-xR′x;
- wherein:
- n is in the range of 0 to 100;
- x is in the range of 1 to 3;
- each of the R groups is selected from the group consisting of methyl, ethyl, propyl, isopropyl, and butyl alkyl;
- and
- each R′ is selected from the group consisting of methyl, ethyl, propyl, isopropyl, butyl, octyl, hexadecyl, octadecyl, phenyl, trimethylaminopropyl, aminopropyl, aminobutyl, methylphosphonyl, hydroxymethal, acetoxymethyl, acetoxypropyl, acetoxypolyethyleneoxypropyl, benzyl, carboxymethoxyethyl, expoxyhexyl, glycidoxypropyl, isocyanatopropyl, and phenethyl;
- and
- wherein the one or more organo-alkoxysiloxane monomers are at an amount that is greater than 0 mol % and no more than about 30 mol % of the siloxane monomer component on a silicon mol basis.
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Dependencies are inferred from the claim text.