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US 2024/0101615 A1Published application
Orthogonal protein heterodimers
AssigneeUniversity of Washington
InventorsZibo CHEN, Scott Boyken, Sherry Bermeo, Robert A. Langan, David Baker
Life of this patent
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Abstract
Disclosed herein are designed heterodimer proteins, monomeric polypeptides capable of forming heterodimer proteins, protein scaffolds including such polypeptides, and methods for using the heterodimer proteins and subunit polypeptides for designing logic gates.
Claims23
1-116. (canceled)
A composition comprising a designed heterodimer protein, wherein the designed heterodimer protein comprises:
- (a) a first polypeptide, wherein the first polypeptide is a non-naturally occurring polypeptide comprising 1-5 alpha helices connected by amino acid linkers;
- and
- (b) a second polypeptide, wherein the second polypeptide is a non-naturally occurring polypeptide comprising 1-5 alpha helices connected by amino acid linkers,
- wherein the first polypeptide and the second polypeptide non-covalently interact to form the designed heterodimer protein,
- wherein the first polypeptide, the second polypeptide, or both are independently linked to one or more:
- effector polypeptide domains, therapeutic agents, or detection tags at the N-terminus, the C-terminus, or both.
3 more elements
A method, comprising:
- (a) detecting heterodimer formation between:
- 1) a first polypeptide, wherein the first polypeptide is a non-naturally occurring polypeptide comprising 1-5 alpha helices connected by amino acid linkers linked to a first polypeptide domain capable of binding to a first target;
- and
- 2) a second polypeptide, wherein the second polypeptide is a non-naturally occurring polypeptide comprising 1-5 alpha helices connected by amino acid linkers linked to a second polypeptide domain capable of binding to a second target;
- and
- (b) detecting binding between the first polypeptide domain and the first target, or detecting binding between the second polypeptide domain and the second target.
3 more elements
Dependencies are inferred from the claim text.