Vlp enteroviral vaccines
AssigneeModerna TX, Inc.
InventorsBrooke Bollman, Gaby Baldeon Vaca, Angelo Cucuzza, Yen-Ting Lai
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Abstract
Aspects of the disclosure relate to compositions of messenger RNA vaccines and methods of administration thereof. Compositions provided herein include one or more RNA polynucleotides having an open reading frame encoding a picornavirus capsid polyprotein and a protease. Compositions provided herein include one or more RNA polynucleotides having an open reading frame encoding an active product that modulates the expression, structure or function of at least one other RNA or product thereof.
Claims64
A composition comprising:
- (i) a messenger ribonucleic acid (mRNA) comprising an open reading frame (ORF) encoding a picornavirus capsid polyprotein, wherein the capsid polyprotein comprises a viral P1 precursor polyprotein;
- and
- (ii) a lipid nanoparticle (LNP).
A composition comprising:
- (i) a messenger ribonucleic acid (mRNA) comprising an open reading frame (ORF) encoding a protease, wherein the protease is a picornavirus 3C protease;
- and
- (ii) a lipid nanoparticle (LNP).
An immunogenic composition comprising:
- (i) a messenger ribonucleic acid (mRNA) comprising an open reading frame (ORF) encoding a picornavirus 3C protease;
- (ii) an mRNA comprising an ORF encoding a picornavirus capsid polyprotein, wherein the capsid polyprotein comprises a viral P1 precursor polyprotein;
- and
- (iii) a lipid nanoparticle (LNP).
The composition of any one of the preceding claims, wherein the lipid nanoparticle comprises 45 mol %, 46 mol %, 47 mol %, 48 mol %, 49 mol %, or 50 mol % ionizable amino lipid.
The composition of any one of the preceding claims, wherein the ionizable amino lipid has the structure of Compound 2:
A method comprising administering to a subject an immunogenic composition comprising:
- (i) a messenger ribonucleic acid (mRNA) comprising an open reading frame encoding a picornavirus protease;
- and
- (ii) a messenger ribonucleic acid (mRNA) comprising an open reading frame encoding a capsid polyprotein comprising a precursor protein, wherein the precursor protein comprises two or more capsid proteins and has a cleavage site specific for the protease between the two or more capsid proteins,
- in an amount effective to induce in the subject an immune response against a viral infection from a member of the Enterovirus genus.
A composition comprising:
- (i) a first messenger ribonucleic acid (mRNA) comprising an open reading frame (ORF) encoding a first product;
- (ii) a second mRNA comprising an ORF encoding a second product;
- and
- (iii) a lipid nanoparticle (LNP),
- wherein the first product is an active protein and wherein the active protein can modulate the expression, structure, or activity of the second mRNA and/or the second product.
Dependencies are inferred from the claim text.