Filovirus Consensus Antigens, Nucleic Acid Constructs And Vaccines Made Therefrom, And Methods Of Using Same

A nucleic acid molecule comprising a nucleotide sequence encoding a consensus Filoviridae envelope glycoprotein immunogen, wherein the nucleotide sequence is selected from the group consisting of:

• a) a nucleic acid sequence that encodes a consensus Zaire ebolavirus envelope glycoprotein immunogen, the amino acid sequence of the consensus Zaire ebolavirus envelope glycoprotein immunogen selected from the group consisting of:
• SEQ ID NO:1 (ZEBOV CON), a fragment of SEQ ID NO:1 that comprises 600 or more amino acids, an amino acid sequence that is at least 95% identical to SEQ ID NO:1, a fragment of an amino acid sequence that is at least 95% identical to SEQ ID NO:1 that comprises 600 or more amino acids;
• SEQ ID NO:1 (ZEBOV CON) linked to an IgE signal peptide, a fragment of SEQ ID NO:1 that comprises 600 or more amino acids linked to an IgE signal peptide, an amino acid sequence that is at least 95% identical to SEQ ID NO:1 linked to an IgE signal peptide, and a fragment of an amino acid sequence that is at least 95% identical to SEQ ID NO:1 that comprises 600 or more amino acids linked to an IgE signal peptide;
• b) a nucleic acid sequence that encodes a consensus Sudan ebolavirus envelope glycoprotein immunogen, the amino acid sequence of the consensus Sudan ebolavirus envelope glycoprotein selected from the group consisting of:
• SEQ ID NO:2 (SUDV CON), a fragment of SEQ ID NO:2 that comprises 600 or more amino acids, an amino acid sequence that is at least 95% identical to SEQ ID NO:2, a fragment of an amino acid sequence that is at least 95% identical to SEQ ID NO:2 that comprises 600 or more amino acids, SEQ ID NO:2 (SUDV CON) linked to an IgE signal peptide, a fragment of SEQ ID NO:2 that comprises 600 or more amino acids linked to an IgE signal peptide, an amino acid sequence that is at least 95% identical to SEQ ID NO:2 linked to an IgE signal peptide, and a fragment of an amino acid sequence that is at least 95% identical to SEQ ID NO:2 that comprises 600 or more amino acids linked to an IgE signal peptide;
• c) a nucleic acid sequence that encodes a consensus Marburg marburgvirus envelope glycoprotein immunogen, wherein the amino acid sequence of the consensus Marburg marburgvirus envelope glycoprotein immunogen is selected from the group consisting of:
• SEQ ID NO:4 (MARV CON1), a fragment of SEQ ID NO:4 that comprises a sequence having 100% identity over 600 or more consecutive amino acids of SEQ ID NO:4, SEQ ID NO:4 (MARV CON1) linked to an IgE signal peptide, and a fragment of SEQ ID NO:4 that comprises a sequence having 100% identity over 600 or more consecutive amino acids of SEQ ID NO:4 linked to an IgE signal peptide;
• d) a nucleic acid sequence that encodes a consensus Marburg marburgvirus envelope glycoprotein immunogen, wherein the amino acid sequence of the consensus Marburg marburgvirus envelope glycoprotein immunogen is selected from the group consisting of:
• SEQ ID NO:5 (MARV CON2), a fragment of SEQ ID NO:5 that comprises a sequence having 100% identity over 600 or more consecutive amino acids of SEQ ID NO:5, an amino acid sequence that is at least 95% identical to the full length of SEQ ID NO:5, a fragment of an amino acid sequence that is at least 95% identical to SEQ ID NO:5 over 600 or more consecutive amino acids, SEQ ID NO:5 (MARV CON2) linked to an IgE signal peptide, a fragment of SEQ ID NO:5 that comprises a sequence having 100% identity over 600 or more consecutive amino acids linked to an IgE signal peptide, an amino acid sequence that is at least 95% identical to the full length of SEQ ID NO:5 linked to an IgE signal peptide, and a fragment of an amino acid sequence that is at least 95% identical to SEQ ID NO:5 that comprises 600 or more consecutive amino acids of SEQ ID NO:5 linked to an IgE signal peptide;
• and
• e) a nucleic acid sequence that encodes a consensus Marburg marburgvirus envelope glycoprotein immunogen, wherein the amino acid sequence of the consensus Marburg marburgvirus envelope glycoprotein immunogen is selected from the group consisting of:
• SEQ ID NO:6 (MARV CON3), a fragment of SEQ ID NO:6 that comprises a sequence having 100% identity over 600 or more consecutive amino acids of SEQ ID NO:6, an amino acid sequence that is at least 95% identical to the full length of SEQ ID NO:6, a fragment of an amino acid sequence that is at least 95% identical to SEQ ID NO:6 over 600 or more consecutive amino acids, SEQ ID NO:6 (MARV CON3) linked to an IgE signal peptide, a fragment of SEQ ID NO:6 that comprises a sequence having 100% identity over 600 or more consecutive amino acids linked to an IgE signal peptide, an amino acid sequence that is at least 95% identical to the full length of SEQ ID NO:6 linked to an IgE signal peptide, and a fragment of an amino acid sequence that is at least 95% identical to SEQ ID NO:6 that comprises 600 or more consecutive amino acids of SEQ ID NO:6 linked to an IgE signal peptide.