Retroviral And Lentiviral Vectors
AssigneeAutolus Limited
InventorsVijay Peddareddigari, Richard Beswick, John Short, Martin Pulé, Ekaterini Kotsopoulou
Life of this patent
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Abstract
The present invention provides a retroviral or lentiviral vector having a viral envelope which comprises a mitogenic T-cell activating transmembrane protein which comprises: (i) a mitogenic domain which binds a mitogenic tetraspanin, and (ii) a transmembrane domain; wherein the mitogenic T-cell activating transmembrane protein is not part of a viral envelope glycoprotein. When cells such as T-cells or Natural Killer cells are transduced by such a viral vector, they are activated by the mitogenic T-cell activating transmembrane protein.
Claims5
1-26. (canceled)
A kit for making a retroviral or lentiviral vector having a viral envelope comprising a mitogenic T-cell activating transmembrane protein, which kit comprises:
- (i) a host cell which expresses, at the cell surface, the mitogenic T-cell activating transmembrane protein,
- (ii) nucleic acids comprising gag, pol, env and optionally rev, and
- (iii) a retroviral or lentiviral genome,
- wherein the mitogenic T-cell activating transmembrane protein comprises:
- (a) a mitogenic domain which binds a mitogenic tetraspanin and
- (b) a transmembrane domain, and
- wherein the mitogenic T-cell activating transmembrane protein is not part of a viral envelope glycoprotein.
A kit for making a retroviral or lentiviral vector having a viral envelope comprising a mitogenic T-cell activating transmembrane protein, which kit comprises:
- (i) a packaging cell which is a host cell that comprises one or more of the genes gag, pol, env and/or rev and that expresses, at the cell surface, a mitogenic T-cell activating transmembrane protein, and
- (ii) a retroviral or lentiviral vector genome,
- wherein the mitogenic T-cell activating transmembrane protein comprises:
- (a) a mitogenic domain which binds a mitogenic tetraspanin and
- (b) a transmembrane domain, and
- wherein the mitogenic T-cell activating transmembrane protein is not part of a viral envelope glycoprotein.
A kit for making a packaging cell, which kit comprises:
- (i) one or more nucleic acid(s) encoding a mitogenic T-cell activating transmembrane protein, and
- (ii) nucleic acids comprising retroviral gag, pol, env and optionally rev genes,
- wherein the mitogenic T-cell activating transmembrane protein comprises:
- (a) a mitogenic domain which binds a mitogenic tetraspanin and
- (b) a transmembrane domain, and
- wherein the mitogenic T-cell activating transmembrane protein is not part of a viral envelope glycoprotein.
A kit for making a producer cell, which kit comprises:
- (i) one or more nucleic acid(s) encoding a mitogenic T-cell activating transmembrane protein,
- (ii) nucleic acids comprising retroviral gag, pol, and env and optionally rev genes,
- (iii) a retroviral or lentiviral vector genome,
- wherein the mitogenic T-cell activating transmembrane protein comprises:
- (a) a mitogenic domain which binds a mitogenic tetraspanin and
- (b) a transmembrane domain, and
- wherein the mitogenic T-cell activating transmembrane protein is not part of a viral envelope glycoprotein.
Dependencies are inferred from the claim text.