Assay Device and Method for Concurrent Separation of Red Blood Cells and a Protein from a Blood Fluid Sample

Abstract

This present disclosure provides devices for analyzing a blood fluid sample. The present disclosure contemplates an assay device that includes a separation membrane and a detection membrane. The separation membrane contains a first reagent for precipitating a first protein contained in the blood fluid sample, where the separation membrane also separates red blood cells from the blood fluid sample. Further, the detection membrane is configured to elicit a quantifiable response in the presence of a second protein present in the blood fluid sample. The quantifiable response corresponds to an amount of the second protein present in the detection membrane. In addition, it is to be understood that the separation membrane concurrently precipitates the first protein and separates the red blood cells from the blood fluid sample that reaches the detection membrane. Methods for manufacturing the assay device and performing an assay using the assay device are also provided.

Dependencies are inferred from the claim text.