Vacuum-Assisted Treatment Device, and Related Systems and Methods

A kit for vacuum-assisted in vivo electroporation of tissue, comprising:

• a housing defining a receptacle and at least one opening into the receptacle;
• at least one port extending through the housing, wherein the at least one port is remote from the at least one opening and is connectable to a vacuum source, such that the at least one port is configured to communicate vacuum pressure from the vacuum source to the receptacle;
• a plurality of inserts that are interchangeably receivable within the receptacle, each insert defining an internal chamber that is in communication with the vacuum source when the insert is received in the receptacle, wherein the plurality of inserts define different respective chamber diameters, each insert including:
• at least one electrode positioned within the chamber, wherein the at least one electrode is configured to deliver one or more electroporation pulses to a targeted portion of tissue extending through the at least one opening and at least momentarily held in the chamber responsive to the vacuum pressure.